摘要
研究了表没食子儿茶素没食子酸酯(EGCG)和茶氨酸两种茶叶主要活性成分单独和协同清除2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)、1,1-二苯基-2-三硝基苯肼(DPPH)和羟自由基的效果,并通过建立细胞氧化损伤模型,测定两种化合物对氧化损伤细胞的氧化水平和抗氧化酶活性的影响。结果表明:EGCG和茶氨酸对ABTS和羟自由基的清除有协同增效作用,对DPPH自由基无协同作用;在细胞水平,EGCG和茶氨酸可协同降低细胞内活性氧自由基及脂质过氧化水平,提高谷胱甘肽过氧化物酶的活性,即两者共同作用可协同降低细胞所受的氧化损伤,提高细胞抗氧化能力。
This paper investigated the scavenging capacities of EGCG and theanine , the two main active components in tea, alone or synergistically on ABTS, DPPH and hydroxyl radicals in vitro were investigated. The oxidative injury experimental model of mouse macrophage （RAW 264.7） by H2O2 was established for measuring the oxidative level and antioxidative enzyme activities by EGCG and theanine. The results showed that EGCG and theanine showed synergistic effect on the scavenging of ABTS and hydroxyl radical except for the DPPH radical. EGCG and theanine could synergistically reduce the contents of reactive oxygen species and lipid peroxidation, and enhance the activity of GSH-Px on RAW 264.7, which meant that the combination of above two compounds could synergistically reduce the oxidative damage of the cells through improving the antioxidant capacity.
出处
《茶叶科学》
CAS
CSCD
北大核心
2014年第3期239-247,共9页
Journal of Tea Science
基金
景宁茶资源生化特征与惠明白茶提高免疫力活性功能研究(H20130018)
浙江大学与Philips茶叶合作项目(I2010007Q)
