摘要
目的:探讨三种不同强度运动对肥胖大鼠肝脏氧化应激及脂联素/脂联素受体等相关指标的影响。方法:经16周高脂饲料喂养构建肥胖大鼠模型,32只模型鼠分为高脂安静组(HF,8只)、小强度运动组(HS组,8只)、中等强度运动组(HM组,8只)和递增负荷运动组(HI组,8只),另外选取正常对照组(NC组)大鼠8只。运动方式采用跑台运动,小强度运动跑速为10 m/min,中等强度跑速为15 m/min,递增负荷强度为10 m/min的速度运动20 min、20 m/min的速度运动20 min、28~30 m/min的速度运动20min。运动组每周运动5天,每次1小时。干预持续6周后检测各组大鼠体重,肝脏超氧化物歧化酶(SOD)活性、丙二醛(MDA)、肿瘤坏死因子α(TNF-α)、白介素6(IL-6)、脂联素受体I(AR1)、AR2,血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、脂联素、甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、自由脂肪酸(FFA)等指标。结果:与NC组比较,HF组大鼠体重、TG、TC、LDL-C、FFA含量、ALT活性、AST活性以及MDA、TNF-α和IL-6表达均显著性升高(P<0.05或P<0.01),HDL-C含量、SOD活性、血清脂联素水平及AR1、AR2表达均显著性降低(P<0.05或P<0.01);与HF组比较,HS、HM和HI组体重、血清TG、LDL-C、FFA含量及肝脏ALT含量、MDA、TNF-α、IL-6表达均显著性降低(P<0.05或P<0.01),SOD活性、脂联素水平及AR1、AR2表达均显著性升高(P<0.05或P<0.01),仅HM组HDL-C含量显著升高(P<0.05);与HS组和HI组比较,HM组FFA含量、MDA表达进一步降低(P<0.05),脂联素水平、AR2进一步增加(P<0.05)。结论:有氧运动能有效改善高脂饮食所致的肥胖大鼠脂代谢异常,提高肝脏抗氧化酶活性,增强自由基清除能力,预防肝细胞炎症损伤,改善肝脏代谢功能紊乱,其中中等强度运动优于小强度或递增负荷强度运动,其机制可能是通过增加血清脂联素进一步激活肝脏脂联素受体2有关。
Objective To discuss effects of three different-intensity exercise on oxidative stress and adiponectin/adiponectin receptor1/2 of the liver in obese rats.Method s Thirty-two Sprague-Dawley rats were fed high-lipid fodder and became the obesity model after 16 weeks.Then they were randomly divided into a high-lipid control group（HF）,a low-intensity exercise group（HS）,a moderate-intensity exercise group（HM） and an exercise group with progressive intensity（HI）,each of 8.Another 8rats were chosen into a normal control group（NC）.The speed of treadmill running of group HS and HM was 10m/min and 15m/min respectively,while that of group HI was10m/min for 20 min,followed by another 20 min at and still another 20 min at 28-30m/min,five days a week for 6 weeks.Six weeks later,the weight,alanine transaminase（ALT）,aspartate transaminase（AST）,superoxide dismutase（SOD）,malondialdehyde（MDA）,tumor necrosis factor alpha（TNF-α）,interleukin-6（IL-6）,adiponectin receptors1/2,adiponectin,triglyceride（TG）,cholesterol（TC）,high-density lipoproteincholesterol（HDL-C）,low-density lipoproteincholesterol（LDL-C） and free fatty acid（FFA） were tested.Results Compared with group NC,significant increase was observed in the average weight,TG,TC,LDL-C,FFA,ALT,AST,MDA,TNF-α and IL-6（P〈0.05 or P〈0.01）,while significant decrease was found in the average HDL-C,SOD,adiponectin and adiponectin receptors1/2 of group HF（P〈0.05 or P〈0.01）.Compared with group HF,there was significant decrease in the average weight,TG,LDL-C,FFA,ALT,MDA,TNF-α and IL-6（P〈0.05 or P〈0.01）,but significant increase in the average SOD,adiponectin,AR1 and AR2 of group HS,HM and HI（P〈0.05 or P〈0.01）,and only HDL-C of group HM increased significantly（P〈0.05）.Compared with group HS and HI,significant decrease was found in the average FFA and MDA（P〈0.05）,while significant increase was found in the average adiponectin and AR2 of group HM（P〈0.05）.Conclusion Aerobic exercise can
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2017年第9期779-787,共9页
Chinese Journal of Sports Medicine
基金
广东省科学事业计划项目(2011B031600010)
广东省体育局科研项目(GDSS2016115)
关键词
不同强度运动
氧化应激
脂联素
脂联素受体2
肥胖大鼠
different intensity exercise, oxidative stress, adiponectin, adiponectin receptor 2, obese rats
