摘要
以甜玉米芯为原料,通过细胞破壁的方式将多糖分子从细胞壁或者细胞质中释放,溶解出的多糖经过脱蛋白、脱色、乙醇沉淀等后续处理步骤得粗多糖.在此基础上,将分子修饰技术应用到甜玉米芯多糖研究中,通过羧甲基化进一步对其进行分子修饰和改性.且分别以氢氧化钠溶液浓度、一氯乙酸用量以及反应温度为单因素条件进行羧甲基化修饰,在单因素实验基础上,通过正交试验进一步确定了最适修饰反应条件为,氢氧化钠溶液浓度4.0 mol/L,一氯乙酸用量2.360 g,反应温度45℃.在此条件下得到羧甲基化多糖取代度为0.051 6.
Polysaccharide molecules released from the cell wall or cytoplasm by the way of cell wall with sweet corncobs as raw material. The polysaccharides were obtained by dissolving the polysaccharides after removal of protein and pigment,ethanol precipitation. On this basis,the molecular modification technology applied to sweet corncob polysaccharides and its molecular modification and modification by the carboxymethylation method. The single factor conditions of carboxymethylation modification with sodium hydroxide solution concentration,MCA dosage and reaction temperature as single factor conditions. The optimal reaction conditions of the modification were determined by orthogonal test on the basis of single factor experiment. The concentration of sodium hydroxide solution,the amount of MCA and the reaction temperature was 4. 0 mol/L,2. 360 g and 45 ℃,respectively. The degree of substitution of carboxymethyl polysaccharides was 0. 051 6 under the condition.
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2017年第4期455-459,共5页
Journal of Harbin University of Commerce：Natural Sciences Edition
关键词
甜玉米芯
多糖
羧甲基化
sweet corn cob
polysaccharides
carboxymethylation
