摘要
目的探讨白藜芦醇(Resveratrol,RSV)对肠缺血再灌注损伤的作用及分子机制。方法 30只SD大鼠随机分成假手术组(Sham组)、肠缺血再灌注损伤组(IRI组)和白藜芦醇预处理组(RSV组)。检测各组沉默信息调节因子1(SIRT1)、乙酰化p53(Acetylp53)、p53、bax、bcl-2、凋亡诱导因子(AIF)和TUNEL,观察肠组织病理形态。结果与Sham组相比,IRI组肠组织病理形态改变以及Acetylp53、bax、细胞浆AIF、TUNEL表达明显增加,而SIRT1和bcl-2表达明显降低。与IRI组相比,RSV组肠组织病理形态改变及Acetylp53、bax、细胞浆AIF、TUNEL表达明显减少,而SIRT1和bcl-2表达明显增加。此外,三组之间p53表达差异无统计学意义。结论 RSV可通过抗凋亡减轻肠缺血再灌注损伤,其作用机制与SIRT1/p53信号通路相关。
Objective To explore the effect and molecular mechanism of resveratrol（ RSV） on intestine ischemia reperfusion injury in rats. Methods Thirty SD rats were randomly divided into Sham group,intestinal ischemia reperfusion injury group（ IRI group） and RSV group. The expression level of SIRT1,Acetylp53,p53,bax,bcl-2,TUNEL and the pathological morphology of intestinal tissue were examined. Results Compared with Sham group,the pathological change of intestinal and the expression of Acetylp53,bax,cytoplasm of AIF and TUNEL in IRI group were significantly increased with the decrease of expression of bcl-2 and SIRT1. Compared with IRI group,the pathological change of intestinal and the expression of Acetylp53,bax,cytoplasm of AIF and TUNEL in RSV group were significantly decreased with the increase of expression of SIRT1 and bcl-2. There was no significant difference in the expression of p53 among the three groups. Conclusion RSV can alleviate intestine ischemia reperfusion injury by anti-apoptosis,the mechanism of which is associated with SIRT/p53 signal pathway.
出处
《实用药物与临床》
CAS
2017年第9期996-999,共4页
Practical Pharmacy and Clinical Remedies
