摘要
目的研究复方丹参明目(FDM)对糖尿病性视网膜病变大鼠的影响及作用机制。方法采用大鼠单次尾静脉注射链脲佐菌素(45 mg·kg^(-1))的方法制备糖尿病模型,继续普通饲料喂养14周,再制备成糖尿病性视网膜病变模型。雄性SD大鼠随机分为正常组,模型组,FDM低、中、高剂量组(200,400,800 mg·kg^(-1)),连续灌胃给药2个月。采用酶联免疫法(ELISA)检测血清中血管内皮生长因子(VEGF)、基质金属蛋白酶-2/9(MMP-2/9)、血小板衍生生长因子A/B(PDGF-A/B)、碱性成纤维细胞生长因子(bFGF)和胰岛素样生长因子1(IGF-1)的含量;采用免疫组化法检测视网膜组织中VEGF和血管内皮细胞生长因子受体2(VEGFR-2)的表达;HE染色观察糖尿病性视网膜病变大鼠视网膜病理学形态。结果与正常组比较,模型组大鼠血清VEGF、MMP-2/9、PDGF-A/B、bFGF和IGF-1含量均显著升高(P<0.01),视网膜组织中VEGF、VEGFR-2表达均显著增多(P<0.01)。与模型组比较,FDM中、高剂量组大鼠血清MMP-2/9、PDGF-A、bFGF和IGF-1含量均显著降低(P<0.05,P<0.01),其中FDM高剂量组的VEGF含量亦显著降低(P<0.01);FDM中、高剂量组大鼠视网膜组织中VEGF、VEGFR-2表达量均显著减少(P<0.01)。结论 FDM能显著改善糖尿病大鼠的视网膜病变,可能与通过抑制VEGF/VEGFR-2及其他促血管生成因子的表达有关。
Objective To investigate the effect and mechanism of Formula Danshen Mingmu （FDM） decoction on diabetic retinopathy in rats. Methods Rats were prepared into diabetic retinopathy model by single tail vein injection of streptozotocin. Fifty SD rats were randomly divided into normal group, model group, low-dose, middle-dose and high-dose groups of FDM （200, 400, 800 mg·kg^-1） , 10 rats in each group. Rats in normal group and model group were administered with water; rats in other groups were given different doses of FDM for two months. Then, serum levels of VEGF, MMP-2, MMP-9, PDGF-A/B, bFGF and IGF-1 in all rats were detected by ELISA; VEGF and VEGFR-2 expression of retina were detected by immunohistochemical method, and retinal pathological morphology were observerd by microscope. Results Compared with normal group, serum VEGF, MMP-2, MMP-9, PDGF- A/B, bFGF and IGF-1 levels incresed significantly （P 〈 0.05）, VEGF and VEGFR-2 expression of retina increased significantly （P 〈 0.01 ）; and the blood vessels of retinal ganglion cell layer, inner nuclear layer and outer nuclear layer incresed significantly. Compared with model group, serum levels of VEGF, MMP-2, MMP-9, PDGF-A/B, bFGF and IGF-1 decresed significantly （P 〈 0.05）, VEGF and VEGFR-2 expression of retina decreased significantly （P 〈 0.01 ）, and the number of blood vessels of retina decreased significantly by the treatment of high-dose and middle-dose of FDM. Conclusion FDM could improve and treat retinopathy in diabetic rats.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2017年第5期628-632,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
武汉市卫生和计划生育委员会科研项目(WX09C09)
