摘要
目的通过研究低氧对大鼠血清bFGF、VEGF的表达和肺动脉超微结构的影响,来初步揭示肺动脉高压的形成机制。方法取30只健康雄性SD大鼠,将其随机分成六组:对照组、低氧1天组、低氧7天组、低氧14天组、低氧21天组和低氧30天组。除对照组外,其余五组大鼠分别放在低压氧舱(模拟海拔5000米)1、7、14、21、30天。取各组大鼠血清及肺动脉,运用ELISA法检测大鼠血清中bFGF、VEGF的表达量,通过透射电镜观察肺动脉超微结构。结果与对照组相比,随着低氧时间的延长,大鼠血清bFGF的表达量逐渐升高,变化具有显著性差异(P<0.05);急性低氧时(低氧1天),血清VEGF含量较对照组明显升高,变化具有显著性差异(P<0.05),随着低氧时间的延长(低氧7、14、21、30天),血清VEGF含量较对照组呈逐渐下降趋势。电镜结果显示,低氧可导致肺动脉有不同程度的损伤,主要表现为,血管内皮细胞核染色质聚集、空泡化,甚至内皮细胞脱落;平滑肌细胞内出现核染色质聚集或边集,胞浆内出现空泡,线粒体肿胀,粗面内质网扩张。结论低氧环境中,bFGF的表达明显升高,VEGF的表达在急性缺氧时明显升高随后有下降趋势,表明bFGF参与低氧性病理生理改变的形成,VEGF变化与急性缺氧有关,低氧可导致肺动脉内皮细胞和平滑肌细胞的结构改变。
Objective To investigate the effects of hypoxia on the expression of bFGF, VEGF and the structure of pulmonary artery in rats, and to reveal the mechanism of pulmonary hypertension.Methods Thirty healthy male SD rats were randomly divided into 6 groups：Control group, hypoxia 1 day group, hypoxia 7 day group, hypoxia 14 day group, hypoxia 21 day group and hypoxia 30 day placed in simulated hypoxic chamber of 5 000 meters group.In addition to Control group,the other five groups were above sea level for 1,7,14,21 and 30 days, respectively, and the serum and pulmonary arteries of each group were taken.The expressions of bFGF and VEGF in serum were detected by ELISA, and ultrastructural changes of pulmonary arteries were observed by transmission electron microscope.Results Compared with the control group,the expression of bFGF in serum increased gradually with the prolongation of hypoxia time（P〈0.05）,the expression of VEGF in acute hypoxia group was significantly higher than that in control group（ P〈0.05 ）, with the prolongation of hypoxia time（ 7,14,21,30 days）, serum VEGF levels were decreased compared with the control group.Electron microscopic results showed that hypoxia can lead different de- grees of pulmonary artery damage, mainly for the endothelial cell nuclear chromatin aggregation, endothelial cell vac- uoles, endothelial cell shedding. Smooth muscle cells, nuclear chromatin aggregation or edge set, cytoplasmic vacu- oles, mitochondrial swelling, rough endoplasmic reticulum expansion, etc. Conclusion In hypoxia environment, the expression of bFGF is increased, the expression of VEGF is increased in acute hypoxia and then decreased, the re- suits show that bFGF is involved in the formation of hypoxic pathophysiological changes.The changes of VEGF are related to the regulation of acute hypoxia. Hypoxia can lead to the structural changes of pulmonary artery endothelial cells and smooth muscle ceils.
作者
李小静
刘川川
刘辉琦
刘杰
曹学锋
吴穹
王生兰
LI Xiao-jing LIU Chuan-chuan LIU Hui-qi LIU Jie CAO Xue-feng WU Qiong WANG Sheng-lan(Department of Pathophysiology, Qinghai University Medical College Affiliated Hospital of Qinghai University, Xining, Qinghai, 810001, China)
出处
《中国高原医学与生物学杂志》
CAS
2017年第3期164-170,共7页
Journal of Chinese High Altitude Medicine & Biology
基金
国家自然科学基金(81160031)
关键词
BFGF
VEGF
低氧
透射电镜
bFGF VEGF Hypoxia Transmission electron microscopy
