摘要
目的观察白藜芦醇(resveratrol,Res)对D-半乳糖(D-galactose,D-gal)诱导的小鼠血清同型半胱氨酸(homocysteine,Hcy)和氧化应激相关指标的影响。方法 48只小鼠随机分为4组:基础对照组、衰老模型组、Res低剂量组(22.5 mg/kg)、Res高剂量组(45 mg/kg),基础对照组每日经颈部皮下注射生理盐水,其余三组经颈部皮下注射D-gal溶液。同时,基础对照组和衰老模型组每日经灌胃给予羧甲基纤维素钠溶液,而其余2组每日经灌胃给予Res羧甲基纤维素钠溶液。16周后检测小鼠血清Hcy水平;血清和肝脏中超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、总抗氧化能力(total antioxidant capacity,T-AOC)和丙二醛(malondialdehyde,MDA)水平。结果与基础对照组相比,衰老模型组小鼠血清Hcy水平显著升高(P<0.05),且其血清和肝脏SOD、GSH-Px和T-AOC显著降低(P<0.05),而MDA水平则显著升高(P<0.05),表明造模成功。与衰老模型组比较,两种剂量的Res均可显著降低小鼠血清Hcy水平(P<0.05),显著升高其血清和肝脏SOD、GSH-Px和T-AOC水平(P<0.05),而显著降低其血清和肝脏MDA水平(P<0.05)。结论不同剂量的Res能够预防D-gal诱导的小鼠氧化应激水平和血清Hcy水平升高,有一定的抗衰老作用。
Objective To study the effect of resveratrol （ Res ） on the levels of homocysteine （ Hcy ） and indices of oxidative stress in aging mice induced by D-galactose （ D-gal ）. Methods Forty-eight mice were divided into 4 groups ： control group, aging model group, Res low dose （22.5mg/kg） group and Res high dose（45mg/kg） group. The mice in the control group were given daily subcutaneous injection with saline, and those in the other three groups were given daily subcutaneous injection with D-gal. Moreover, the mice in the control group and aging model group were given sodium carboxymethyl cellulose solution, and the mice in the Res low dose group and Res high dose group were given Res dissolved in sodium carboxymethyl cellulose solution. After 16 weeks, homocysteine （Hcy） level in serum, superoxide dismutase（SOD） , glutathione peroxidase（GSH-Px）, total antioxidant capacity （ T-AOC ） and malondialdehyde （ MDA ） levels in serum and liver of mice were measured. Results Compared with the mice in the control group, the levels of Hcy in serum and MDA in serum and liver of the mice in the aging model group were significantly increased （P〈0.05） , while the levels of SOD, GSH-Px and T-AOC in serum and liver were significantly decreased （ P〈 0.05） , suggesting the aging model was successful. Compared with the mice in the aging model group, administration of Res at low and high doses significantly lowered the levels of Hcy in serum and MDA in serum and liver, and raised the levels of SOD, GSH- Px and T-AOC in serum and liver. Conclusions Different doses of Res can prevent the increase of oxidative stress and Hcy induced by D-gal in mice. Res has anti-aging effect.
作者
任亚浩
李翠
赵越
杨军
REN Ya-hao LI Cui ZHAO Yue YANG Jun(Department of Nutritional and Food Hygiene, School of Public health, China Medical University, Shenyang,Liaoning 110122, China)
出处
《实用预防医学》
CAS
2017年第10期1172-1174,共3页
Practical Preventive Medicine
基金
辽宁省教育厅高校科研计划项目(2009A720)
