摘要
目的 检测膜联蛋白A10（ANXA10）在人肝细胞癌（HCC）组织中的表达水平,分析其与基质金属蛋白酶-9（MMP-9）和血管内皮生长因子（VEGF）的相关性,为临床诊断、评估HCC提供依据.方法 选择HCC患者88例,均进行手术治疗,HCC癌组织Ⅰ期11例、Ⅱ期25例,Ⅲ期31例、Ⅳ期21例,应用免疫组织化学法检测患者HCC癌组织、癌旁肝组织和正常肝组织中ANXA10、MMP-9、VEGF的表达,分析ANXA10与MMP-9、VEGF之间相关性.结果 HCC癌组织ANXA10吸光度值为（0.074±0.012）,低于癌旁肝组织的（0.091±0.013）和正常肝组织的（0.131 ±0.025）,且癌旁肝组织ANXA10表达低于正常肝组织,差异均有统计学意义（t=8.96、9.44、8.71,均P＜0.05）;HCC癌组织MMP-9和VEGF吸光度值分别为（0.147 ±0.017）和（0.127 ±0.028）,高于癌旁肝组织的（0.096 ±0.012）和（0.091 ±0.015）、正常肝组织的（0.075±0.014）、（0.077 ±0.019）,且癌旁肝组织MMP-9和VEGF表达高于正常肝组织,差异均有统计学意义（t=8.05、9.30、8.11,8.28、9.51、8.02,均P＜0.05）;Ⅲ期＋Ⅳ期HCC癌组织ANXA10吸光度值为（0.056 ±0.010）,低于Ⅰ期＋Ⅱ期的（0.082 ±0.016）,差异有统计学意义（t=8.90,P＜0.05）,Ⅲ期＋Ⅳ期HCC癌组织MMP-9和VEGF吸光度值为（0.157±0.022）和（0.169±0.033）,高于Ⅰ期＋Ⅱ期的（0.114±0.015）和（0.091±0.021）,差异均有统计学意义（t=9.13、9.72,均P＜0.05）.ANXA10与MMP-9和VEGF具有显著相关性（r=0.324,0.295,均P＜0.05）.结论 ANXA10在肝细胞癌组织中呈现低表达,并随着分期的增加表达下降,与MMP-9和VEGF呈现负相关,ANXA10表达缺失或失活可能是恶变的人肝细胞癌的重要特征之一.
Objective To explore the expression of annexin A10 （ANXA10） in human hepatocellular carcinoma （HCC）,and analyze its correlation with matrix metalloproteinases-9 （MMP-9） and vascular endothelial growth factor （VEGF）,thus to provide the basis for clinical diagnosis and assessment of HCC.Methods 88 HCC patients were selected,and they were all performed surgical treatment,HCC stage Ⅰ in 11 cases,stage Ⅱ 25 cases,stage Ⅲ 31 cases,stage ⅣV 21 cases.The expressions of ANXA10,MMP-9,VEGF of HCC cancer tissues,adjacent liver tissues and normal liver tissues were tested by immunohistochemical method.The correlation of ANXA10 with VEGF and MMP-9 was analyzed.Results The absorbance value of ANXA10 expression in the HCC cancer tissue was （0.074 ± 0.012）,which was lower than that in the adjacent liver tissues [（0.091 ± 0.013）] and normal liver tissues[（0.131 ±0.025）],and ANXA10 expression of the adjacent liver tissue was lower than that of the normal liver tissues,the differences were statistically significant （t =8.96,9.44,8.71,all P 〈 0.05）.The absorbance values of MMP-9 and VEGF expression in the HCC cancer tissue were （0.147 ± 0.017） and （0.127 ± 0.028）,respectively,which were higher than those in the adjacent liver tissues [（0.096 ± 0.012）,（0.091 ± 0.015）] and normal liver tissues [（0.075 ± 0.014）,（0.077 ± 0.019）].The absorbance values of MMP-9 and VEGF of the adjacent liver tissues were higher than those of the normal liver tissues,the differences were statistically significant （t =8.05,9.30,8.11;8.28,9.51,8.02,all P 〈 0.05）.The absorbance value of ANXA 10 expression in the HCC stage Ⅲ ＋ Ⅳ cancer tissue was （0.056 ± 0.010）,which was lower than that in the stage Ⅰ ＋ Ⅱ cancer tissue [（0.082 ±0.016）],the difference was statistically significant （t =8.90,P 〈 0.05）.The absorbance values of MMP-9 and VEGF expression in the stage Ⅲ ＋ Ⅳ cancer tissue were （0.157 ± 0.022） and （0.169 ± 0.033）,respectively,whi
出处
《中国基层医药》
CAS
2017年第20期3058-3061,共4页
Chinese Journal of Primary Medicine and Pharmacy
基金
浙江省温岭市科技计划项目（W081057）
