摘要
试验旨在建立一种同时检测鸡蛋中5种青霉素类药物残留的高效液相色谱紫外检测法。鸡蛋经乙腈提取、正己烷脱脂、旋转蒸发浓缩和衍生化后,采用高效液相色谱法分离检测。结果显示,氨苄西林(ampicillin,AMP)、氯唑西林(cloxacillin,CLO)、苯唑西林(oxacillin,OXA)、阿莫西林(amoxicillin,AMO)及普鲁卡因青霉素(penicillins G procaine,PRO)在10~1 000μg/kg呈良好线性关系,R^2>0.999;检测限为1.5μg/kg(S/N=3),定量限为5.0μg/kg(S/N=10),添加平均回收率为62.04%~93.13%,相对标准偏差为3.91%~13.69%,批内相对标准偏差为3.98%~14.71%,批间相对标准偏差为4.26%~12.71%。表明本试验建立的高效液相色谱紫外法重复性和灵敏度较高,适用于鸡蛋中的5种青霉素类药物残留检测。
This study was aimed to develop a method for simultaneous determination of five kinds of penicillins in eggs by high performance liquid chromatography-ultraviolet detection（HPLCUVD）.The eggs samples were extracted by acetonitrile,degreased by n-hexane,concentrated by rotary evaporation,derivatization,and then was separated by HPLC.The results showed that the calibration curves of five kinds of penicillins were linear correlated in the concentration ranges of10 to 1 000μg/kg with the correlation coefficients 0.999,which the limits of detection and quantification were 1.5μg/kg（S/N=3）and 5.0μg/kg（S/N=10）,respectively.The average recoveries were 62.04%to 93.13%,the relative standard deviations were 3.91%to 13.69%.And the intra-day and inter-day RSDs were 3.98% to 14.71% and 4.26% to 12.71%,respectively.The developed method was suitable for detecting five kinds of penicillins residues in eggs with high repeatability and sensitivity.
出处
《中国畜牧兽医》
CAS
北大核心
2017年第8期2483-2488,共6页
China Animal Husbandry & Veterinary Medicine
基金
基金项目:农业行业标准制定和修订(SYJ-58-359)
关键词
青霉素
高效液相色谱紫外检测法
药物残留
鸡蛋
penicillin
high performance liquid chromatography-ultraviolet detection（HPLC-UVD）
drug residues
eggs
