摘要
样品采用乙腈直接提取，离心分离及固相（Ｃ１８柱）净化方式，经衍生后，用液相色谱紫外检测器测定牛奶中５种青霉素残留量。方法最小检测质量浓度０．００５ｍｇ／Ｌ，回收率范围在６８．９％～１０１．３％之间，相对标准偏差为３．２１％～６．１８％。
A high performance liquid chromatographic method with UV detection was developed for ana- lysis of penicillin G, penicillin V, oxacillin, cloxacillin, dicloxacillin in milk. The milk sample was treated by extraction with acetonitrile, followed by centrifugation precipitation of proteins and solid-phase (C18) purification, after derivatization of the 5 penicillins with 1, 2, 4-triazole and HgCl2 under 60℃ for 10 min, the products of derivatization were determined by reverse-phase LC with UV detection. The minimum detectable mass concentration were 0.005 mg/L. Average recoveries of 5 penicillins were in the range of 68.9% - 101. 3% and relative standard deviations 3.21 % - 6. 18%.
出处
《分析测试学报》
CAS
CSCD
2000年第6期72-74,共3页
Journal of Instrumental Analysis
