摘要
以金铁锁组培苗根尖为材料,对其染色体制片过程中预处理、固定、解离、染色等环节的最佳处理条件进行探讨。结果表明,金铁锁根尖在室温下用0.003 mol/L 8-羟基喹啉溶液预处理5.0 h后,放入预冷的卡诺固定液[V(无水乙醇)∶V(冰乙酸)=3∶1]中于冰水中处理30 min,然后用解离液[V(浓HCl)∶V(无水乙醇)=1∶1]处理120 s,再用卡宝品红染色液对根尖染色25 min(先染色20 min后复染5 min),能得到良好的镜检效果。金铁锁根尖体细胞的染色体数目为2n=2x=28,为二倍体。
The root tips of tissue culture seedlings of Psammosilene tunicoides were used as materials to find the optimum treatment condition in steps of preculture, fixing, dissociation, and dyeing in slice pro-duction process. The results showed that the good microscopic images would be obtained when the root tip of P. tunicoides was pretreated with 0. 003 mol/L 8-hydroxyquinoline solution for 5.0 hours at room tem-perature, then put into the Carnoy solution [ F （ anhydrous ethanol） ： F （ glacial acetic acid） =3：1] and treated with ice water for 30 min, afterwards treated with dissociation solution [ F （ concentrated HC1） ： V （anhydrous ethanol） =1：1] for 120 s , and finally dyed with Carbol fuchsin for 25 min （dyed for 20 min and redyed for 5 min）. The chromosome number of somatic cell in P. tunicoides root tips is 2n = 2 x =28. It is diploid plant.
出处
《河南农业科学》
CSCD
北大核心
2017年第8期111-114,120,共5页
Journal of Henan Agricultural Sciences
基金
云南省教育厅科学研究基金项目(2016ZZX152)
贵州省社发公关项目(黔科合SY字[2015]3027号)
云南省林学一级学科博士点建设项目
西南林业大学科研基金项目(XL21611)
关键词
金铁锁
染色体
制片技术
Psammosilene tunicoides
chromosome
slice technique
