摘要
目的优化三白草酮超临界CO2萃取工艺,并评价三白草Saururi Herba超临界萃取物体外抗肿瘤活性。方法以萃取压力、萃取温度、萃取时间、夹带剂(乙醇)体积分数、夹带剂用量为影响因素,三白草酮提取率为评价指标,正交试验优化萃取工艺,MTT法测定萃取物对人肝癌细胞7721/Adm耐药株的抑制作用。结果最佳条件为萃取压力30 MPa,萃取温度50℃,萃取时间2 h,乙醇体积分数95%,用量1倍,三白草酮平均提取率0.173%。所得萃取物显著抑制了7721/Adm细胞增殖(IC50=50.08μg/mL),活性强于乙醇提取物(IC50=150.59μg/mL)。结论该方法稳定可行,适合萃取三白草酮,而且三白草超临界CO2萃取物具有较强的体外抗肿瘤活性。
AIM To optimize the supercritical CO_2 extraction of sauchinone and to evaluate the in vitro antitumor activity of Saururi Herba supercritical extract. METHODS With extraction pressure,extraction temperature,extraction time,entrainer（ ethanol） concentration and entrainer amount as influencing factors,together with extraction rate of sauchinone as an evaluation index,orthogonal test was used for optimizing the extraction. Then MTT was applied to determining the extract＇s inhibitory effect on human multidrug-resistant hepatocellular carcinoma cell line（ 7721/Adm）. RESULTS The optimal conditions were determined to be 30 MPa for extraction pressure,50 ℃ for extraction temperature,2 h for extraction time,95% for ethanol concentration,and one time for ethanol amount,the average extraction rate of sauchinone was 0. 173%. The obtained extract significantly inhibited the proliferation of 7721/Adm cells（ IC50= 50. 08 μg/m L）,demonstrating a stronger activity than that of ethanol extract（ IC50= 150. 59 μg/m L）. CONCLUSION This stable and feasible method is appropriate for sauchinone extraction,and the supercritical CO2 extract from Saururi Herba shows a strong in vitro antitumor activity.
出处
《中成药》
CAS
CSCD
北大核心
2017年第8期1601-1604,共4页
Chinese Traditional Patent Medicine
基金
浙江省自然科学基金项目(LQ15H280006)
浙江省教育厅科研项目(Y201226181)
宁波市自然科学基金项目(2013A610271)
浙江医药高等专科学校校级科研重点项目(ZPCSR2014001)
