摘要
目的探讨可溶性E-钙黏蛋白(soluble E-cadherin,SE-CAD)、可溶性E选择素(soluble E-selectin,SESEL)、可溶性细胞间粘附分子1(soluble Intercellular adhesion molecule-1,s ICAM-1)在肺癌临床诊断中的价值。方法选取2011年6月~2014年12月在本院确诊的168例患者,均经组织学确诊为肺癌,其中小细胞肺癌(small cell lung cancer,SCLC)98例、非小细胞肺癌(non-small cell lung cancer,NSCLC)70例,肺部良性疾病患者和健康自愿者各50例。所有患者采集静脉血分离血清,采用抗SE-CAD、抗SE-SEL和抗s ICAM-1的单克隆抗体试剂盒ELISA方法测定血清粘附分子。结果 SCLC和NSCLC患者血清SE-CAD、SE-SEL和s ICAM-1水平显著高于健康组和良性疾病组(P﹤0.05),而且肿瘤发生转移(III^IV期)的患者血清SE-CAD水平高于无转移(I^II期)的患者(P﹤0.05),但SE-SEL与s ICAM-1与是否发生转移没有相关性。结论 SE-CAD、SE-SEL和s ICAM-1对肺癌临床诊断具有一定价值,而且SE-CAD可作为判断肿瘤是否扩散的一个有用指标,从而进行疗效观察和愈后评估。
Objective In the present study,we investigated the role of soluble E-cadherin （SE-CAD）,soluble E-selectin （SE-SEL） and soluble intercellular adhesion molecule-1 （sICAM-1） as biomarkers in lung cancer. Methods 168 patients with recently diagnosed lung cancer,98 with small cell lung cancer（SCLC）,and 70 with non-small cell lung cancer （NSCLC）,50 cases of patients with benign pulmonary diseases as well as 50 healthy volunteers were enrolled. Blood samples were collected at the time of diagnosis and measurement of soluble adhesion molecules in the serum samples was performed by enzyme-linked immunoassay using monoclonal antibodies against E-cadherin,E-selectin,and ICAM-1. Results Serum levels of SE-CAD,SE-SEL,and sICAM-1 in both SCLC and NSCLC were significantly higher than the healthy subjects and the group with benign pulmonary diseases （P〈0.05）. In addition,patients with SCLC or NSCLC with distant metastasis had a marked increase of SE-CAD（P〈0.05）,but no such correlation with SE-SEL and sICAM-1 was found. Conclusions Our findings suggest that SE-CAD,SE-SEL,and sICAM-1 have an adjunctive diagnostic role in lung cancer. Furthermore,SE-CAD may also have a prognostic value and could be a useful biomarker in the prediction of lung cancer outcome.
出处
《临床输血与检验》
CAS
2017年第4期382-385,共4页
Journal of Clinical Transfusion and Laboratory Medicine
基金
河北省廊坊市科技局项目(No.2014013022)资助
