摘要
目的:观察肝郁脾虚证模型大鼠海马色氨酸羟化酶2(TPH2)与吲哚胺-2,3-双加氧酶1(IDO1)表达的变化并探讨逍遥散的调节作用。方法:雄性SD大鼠随机分成4组,分别是正常组、模型组、逍遥散组、氟西汀组,每组6只。采用慢性束缚应激的方法制备大鼠肝郁脾虚证模型,造模持续21 d。通过荧光定量PCR与Western-blot方法检测各组大鼠海马TPH2与IDO1的表达情况。结果:模型组大鼠海马TPH2 mRNA的表达水平低于其余3组大鼠(P<0.05),逍遥散与氟西汀对TPH2 mRNA的表达有一定的上调作用;模型组大鼠海马IDO1 mRNA的表达显著增加(P<0.01),逍遥散与氟西汀对IDO1 mRNA表达的下调作用明显(P<0.01),且逍遥散对IDO1 mRNA的下调作用更明显。模型组、逍遥散组、氟西汀组大鼠海马TPH2的蛋白的表达低于正常组(P<0.01),逍遥散组TPH2蛋白表达高于模型组(P<0.05);模型组大鼠海马IDO1的蛋白表达显著高于其余3组(P<0.01)。结论:肝郁脾虚证模型大鼠海马TPH2的表达减少,IDO1的表达增多,逍遥散可能通过调节海马TPH2与IDO1的表达水平进而影响5-HT的含量,起到治疗作用。
Objective：To observe the expression of TPH2 and IDOl in hippocampus of rat with syndrome of liver depression and spleen deficiency, and to explore the regulating effect of xiaoyaosan. Methods：Male SD rats were randomly divided into 4 groups： normal group, model group, xiayaosan group and fluoxetine group, each including 6 rats. The liver depression and spleen deficien- cy model was established by chronic immobilization stress for 21 days. The expression of TPH2 and IDOl were detected by Fluo- rescent quantitative PCR and Western-blot method. Results：The mRNA expression of TPH2 in hippocampus of model group de- creased significantly compared with the other groups （ P 〈 0. 05 ） ; xiaoyaosan and fluoxetine could increase the TPH2 mRNA ex- pression. The mRNA expression of IDOl in hippocampus of model group increased significantly （P 〈 0. 01 ） ; xiaoyaosan and flu- oxetine had the down-regulatory effect on IDOl mRNA expression （ P 〈 0. 01 ） , which is more obvious in xiaoyaosan group. The protein expression of TPH2 in normal group was higher than the other groups （ P 〈 0. 01 ） , which of xiaoyaosan group was higher than model group （ P 〈 0. 05 ） ; the protein expression of IDOl in model group increased significantly compared with the other groups （ P 〈 0. 01 ）. Conclusion ： The expression of TPH2 decreases and IDOl increases in liver depression and spleen deficiency model rats, and xiaoyaosan has a therapeutic effec by regulating TPH2 and IDOl in hippocampus to influence the content of 5-HT.
出处
《世界中医药》
CAS
2017年第3期494-498,共5页
World Chinese Medicine
基金
国家自然科学基金面上项目(编号:81473597)
国家自然科学基金重点项目(编号:81630104)
北京中医药大学自主课题(编号:2016-JYB-XS012)
关键词
肝郁脾虚证
逍遥散
TPH2
IDO1
Syndrome of liver depression and spleen deficiency
Xiaoyaosan
TPH2
IDO1
