摘要
以金针菇为研究材料,通过液体发酵技术获得富锗金针菇菌丝体,对富锗金针菇菌丝体多糖进行了分离和纯化,通过体外抗氧化试验测定了富锗金针菇多糖对自由基的清除能力。试验表明:富锗金针菇菌丝体经提取纯化后得到单一组分多糖,纯度为99.78%,不含蛋白质;富锗金针菇菌丝体锗含量为7.42 mg/g,有机锗的转化率为6.25%;富锗金针菇多糖经纯化后抗氧化性显著增强,对氧自由基、羟自由基和DPPH自由基均具有较好的清除作用,与空白对照相比均具有显著差异,自由基清除率随富锗多糖的浓度增加而增强。在试验范围内当富锗多糖浓度为900μg/mL时,对氧自由基、羟自由基和DPPH自由基的清除率最高分别达68.16%、64.26%和64.3%。该富锗多糖具备开发为天然抗氧化剂、功能性食品或药品的潜力。
Flammulina velutipes strain was used as research material,germanium enriched hyphae was obtained by fermentation technology,and polysaccharides were separated and purified by DEAE cellulose and G-100 chromatography column. Antioxidant activity of germanium enriched Flammulina velutipes polysaccharide in vitro was studied. Experimental results showed that,a single component polysaccharide was obtained by separation and purification,purity 99. 78%,nonprotein. Ge content in mycelium was 7. 42 mg/g,percent conversion of organic Ge was 6. 25%. Antioxidant ability enhanced significantly of polysaccharide purified and effectively scavenged oxygen free radical,hydroxyl radical and DPPH,they had significant difference compared with blank control. Scavenging rate of free radical increased with concentration of polysaccharide Ge enriched. When the polysaccharide concentration was 900 μg/mL,scavenging rate of oxygen free radical,hydroxyl radical and DPPH was 68. 16%,64. 26%,64. 3%,respectively. The polysaccharides Ge-riched had potential to developed into a natural antioxidant,functional foods or drugs.
出处
《农业工程》
2017年第3期100-104,112,共6页
AGRICULTURAL ENGINEERING
基金
2015年国家大学生实践创新项目(项目编号:201511998004Z)
2012年徐州工程学院科研计划项目(项目编号:XKY2012214)
2014年江苏省前瞻性研究专项资金项目(项目编号:苏财教[2014]108号)
关键词
金针菇
锗
多糖
自由基
Flammulina velutipes
Germanium
Polysaccharide
Free radicals
