摘要
为探讨谷胱甘肽转移酶(GST)的抗逆机理,克隆木豆中的抗旱谷胱甘肽转移酶基因,基于木豆干旱胁迫c DNA文库中1个GST基因相关的EST片段,利用RACE技术从木豆幼苗中克隆了该基因,命名为CcGST1,研究其在不同组织及不同干旱时期的表达特性。结果表明:该基因含1个669 bp的开放阅读框,编码223个氨基酸残基,含有保守的谷胱甘肽-S-转移酶的N端和C端结构域;系统进化树分析结果表明木豆CcGST1与大豆、宽叶菜豆等豆科植物的GST亲缘关系较近;亚细胞定位预测CcGST1蛋白可能定位于叶绿体;荧光定量PCR分析表明,CcGST1在木豆幼苗根、茎和叶中均有表达,但根中的表达水平最高,同时该基因受干旱胁迫诱导表达。据此推测木豆CcGST1基因可能与木豆应激干旱胁迫相关。
One glutathione S.transferase （GST） gene associated with the drought responsiveness was cloned from Cajanus cajan to explore the stress resistance mechanism by rapid amplification of cDNA ends. It was designated CcGST1 and contained an open reading frame of 669bp, which encodes 223 amino acid residues. It also contained conserved glutathione S.transferase N- and C-terminal domain. Phylogenetic analysis revealed that CcGST1 was closely related to GST from other legume plants, such as Glycine max and Phaseolus aculifolius. Subcellular localization analysis indicated that CcGST1 was targeted to the chloroplast. By fluorescent quantitative real-time PCR, CcGST1 mRNA transcripts were detectable in all tissues, with the highest transcriptional level found in the root. Furthermore, the expression of CcGST1 transcript was markedly induced by drought stimulation. The result suggested that the CcGST1 might be involved in drought response in C.cajan.
作者
乔光
文晓鹏
洪怡
Qiao Guang Wen Xiaopeng Hong Yi(Collabrative Innovation Center for Mountain Ecology ＆ Agro-bioengineering （CICEAB）, Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region （ Ministry of Education） , Institute of Agro-bioengineering, Guizhou University, Guizhou Guiyang 550025, Chin)
出处
《西南林业大学学报（自然科学）》
CAS
北大核心
2017年第4期1-7,共7页
Journal of Southwest Forestry University：Natural Sciences
基金
国家自然科学基金项目(30860224)资助
贵州省农业攻关项目(20092076)资助
关键词
木豆
谷胱甘肽转移酶
克隆
基因
生物信息学
表达分析
Cajanus cajan, glutathione S-transferase （GST）, colone, gene, bioinformatics, expression analysis
