摘要
毛竹Phyllostachys edulis是中国主要的经济竹种,纤维素合成对于竹材的形成具有重要意义。纤维素主要由纤维素合成酶(CesA)合成,并储存在植物的初生壁和次生壁中。通过生物信息学方法、透射电镜观察以及荧光定量聚合酶链式反应(qRT-PCR)技术研究了毛竹纤维素合成酶的表达和功能。共获得16个毛竹纤维素合成酶家族基因。结构域分析表明:毛竹纤维素合成酶都含有cellulose_synt结构域,N端大多有锌(Zn)指结构。毛竹韧皮部细胞超微结构观察发现:次生壁随着高的增加而加厚,次生壁的初始形成在上升期(1.2m)。定量分析表明:8个基因(PeCesA1,PeCesA2,PeCesA3,PeCesA4,PeCesA5,PeCesA6,PeCesA9和PeCesA13)在次生壁形成的部位表达最显著,3个基因(PeCesA6,PeCesA9和PeCesA13)在初生壁结构部位表达最显著。结合前人对相同时期毛竹的纤维素含量研究,推测在毛竹幼竹生长过程中PeCesA1,PeCesA2,PeCesA3,PeCesA4和PeCesA5基因主要参与毛竹次生壁的合成,PeCesA6,PeCesA9和PeCesA13对初生壁和次生壁的形成都起到一定作用。
Phyllostachys edulis, a major commercial bamboo species in China, utilizes cellulose synthesis,which mainly synthesizes cellulose deposited in the primary and secondary cell walls and is key in bamboo growth and development. This study was conducted to determine the structure and function of cellulose synthase as it promotes bamboo growth and development. Expression and function of cellulose synthase were researched with a bioinformatics analysis, transmission electron microscope（TEM）, and quantitative real-time polymerase chain reaction（qRT-PCR）. Results indicated that 16 members of the cellulose synthase family were predicted, and the proteins contained a cellulose-synt motif and a zinc finger motif on the N terminus using domain prediction. Cell phloem ultrastructures of Ph. edulis showed that the secondary cell wall was thicker with an increasing height of bamboo, and first appeared in the basal part（first 1.2 m）. Eight cellulose synthase genes（PeCesA1, PeCesA2, PeCesA3, PeCesA4, PeCesA5, PeCesA6, PeCesA9, and PeCesA13）had a high expression on parts of the secondary cell wall; whereas, three cellulose synthase genes（PeCesA6, PeCesA9, and PeCesA13） had a high expression on parts of the primary cell wall. Based on previous studies of bamboo cellulose content for the same growth stages, for young bamboo, PeCesA1, PeCesA2, PeCesA3, PeCesA4, and PeCesA5 were the main genes responsible for the secondary cell wall; and PeCesA6, PeCesA9, and PeCesA13 were likely involved in building both primary and secondary cell walls.
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2017年第4期565-573,共9页
Journal of Zhejiang A&F University
基金
国家自然科学基金资助项目(31570686)
浙江农林大学人才启动基金资助项目(2012FR079)
关键词
植物学
毛竹
纤维素合成酶
韧皮部
超微结构
初生壁
次生壁
botany
Phyllostachys edulis
cellulose synthase
phloem
ultrastructure
primary cell wall
secondary cell wall
