摘要
土壤盐渍化是制约中国农业生产的重要问题,通过对航天诱变的野大豆材料进行耐盐性评价,筛选高耐盐的野大豆材料对盐碱地的开发利用具有重要的意义。本研究对129份野大豆航天诱变SP1苗期材料用150 mmol·L^(-1)浓度的NaCl进行盐胁迫处理,测定苗期株高、下胚轴长、下胚轴粗(茎粗)、叶绿素含量、单叶干鲜重以及叶面积等11个指标,采用主成分分析、耐盐性综合评价分析以及聚类分析等多元统计分析方法对野大豆突变材料进行苗期耐盐性评价。结果表明盐胁迫下野大豆突变材料农艺性状多态性丰富,变异系数范围为6.45%~51.78%,21 d株高、7d株高和叶面积3个指标受盐胁迫影响较大,并且11个性状指标间存在着不同程度的相关性。以5个主成分综合指标为基础获得综合评价指标D值,根据D值筛选到高耐盐野大豆突变材料10份,耐盐材料53份,中间型材料63份以及3份不耐盐材料。生理指标检测发现过氧化物酶活性在野大豆诱变材料耐盐过程中发挥重要作用。鉴定出的耐盐材料为野大豆耐盐育种特别是栽培大豆的耐盐育种提供了优异种质资源。
Soil salinity is one of the major issues affecting agricultural production in many countries,including China.To identify and obtain highly salt-tolerant wild soybean(Glycine soja)genotypes for cultivation in saline-alkali regions,the salt tolerance of spaceflight-mutated wild soybean materials was evaluated.In total,129 SP1 samples of wild soybean that had undergone spaceflight mutagenesis were treated with 150 mmol·L^(-1) NaCl and then 11 indexes were measured.The indexes included plant height,hypocotyl length,hypocotyl diameter(stem diameter),chlorophyll content,single leaf dry and fresh weight,and leaf area.The salt tolerance of wild soybean mutant materials was assessed at the seedling stage using multivariate statistical analyses including principal component analysis and cluster analysis.There were significant variations in agronomic traits among the wild soybean mutant materials.The coefficient of variation of the various traits ranged from 6.45% to 51.78%.The most significant correlations among the 11 traits were found between plant height at 7 days and 21 days and leaf area.Based on the first five principal component scores,the D value representing a comprehensive evaluation index was determined.Ten highly salt-tolerant mutant materials,53 salt-tolerant materials,63 intermediate salt-tolerant materials,and three salt-sensitive materials were screened based on the D value.Peroxidase activity was identified as a crucial factor in the salt tolerance of wild soybean mutant materials.These salt-resistant materials are promising foundational resources for breeding new,salt-tolerant soybean varieties.
作者
徐宗昌
鲁雪莉
魏云冲
孟晨
张梦超
张缘杨
王萌
王菊英
张成省
李义强
XU Zong-chang;LU Xue-li;WEI Yun-chong;MENG Chen;ZHANG Meng-chao;ZHANG Yuan-yang;WANG Meng;WANG Ju-ying;ZHANG Cheng-sheng;LI Yi-qiang(Marine Agriculture Research Center,Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266100,China;College of Agriculture,Qingdao Agricultural University,Qingdao 266109,China;College of Agriculture,Shanxi Agricultural University,Jinzhong 034100,China;National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land,Dongying 257345,China;Qingdao Key Laboratory of Resources Mining and Biological Breeding in Coastal Saline-alkali Land,Qingdao 266100,China)
出处
《草业学报》
CSCD
北大核心
2023年第11期168-178,共11页
Acta Prataculturae Sinica
基金
中央级公益性科研院所基本科研业务费专项(1610232022006)
国家自然科学基金面上项目(32171948)
中国农业科学院科技创新工程(编号CAAS-ZDRW202201)
青岛市科技惠民示范引导专项(20-3-4-7-nsh)资助。
关键词
野大豆
航天诱变
苗期
主成分分析
隶属函数
综合评价
wild soybean
space mutagenesis
seedling stage
principal component analysis
membership function
comprehensive evaluation
