摘要
本研究利用转TaNHX2基因大豆T3代株系,研究其在干旱胁迫情况下表型、生理特性、生物量与光合作用等方面的变化。结果表明:随着干旱时间的不断延长,对照表现出明显的萎蔫,且转基因植株能够持续地保持绿色状态。转基因植株叶片中含水量高于对照,但株系间叶绿素和可溶性糖含量差异不大,转基因植株中的脯氨酸和丙二醛含量明显低于对照,SOD的活性高于对照。综合评价表明转TaNHX2基因大豆T3代株系比对照具有更强的耐旱性。
The TaNHX2 transgenic soybean T3 generation plants were used to study the phenotypic, physiological characteristics, photosynthesis, biomass and other changes under drought stress. The results showed that with the prolongation of drought time, the control was wilting, the transgenic plants kept green, and the water content in the leaves of the transgenic plants was higher than that of the control. However, there was no significant difference of chlorophyll content and soluble sugar in the transgenic plants. Proline and malondialdehyde content of transgenic plants were significantly lower than the control, SOD activity was higher than the control. The comprehensive evaluation showed that the transgenic soybean plants with TaNHX2 gene had obvious drought tolerance compared with the control.
出处
《大豆科学》
CAS
CSCD
北大核心
2017年第4期519-524,共6页
Soybean Science
基金
国家自然科学青年基金(31201229)
黑龙江省普通高等学校青年学术骨干支持计划(1253G008)
黑龙江省科技厅国际科技合作项目(WC05B10)
关键词
大豆
TaNHX2
农杆菌介导法
转基因
耐旱性
Soybean
TaNHX2
Agrobacterium-mediated method
Transgenic
Drought tolerance
