摘要
将Barnase及Barstar基因构建的不育及恢复基因载体,利用农杆菌介导法转入棉花基因组,经田间生物学检测及分子检测,获得120株T0阳性棉花不育系植株和76株T_0阳性棉花恢复系植株。经回交转育,选育成不同性状的不育系材料5个,恢复性良好的恢复系材料3个;获得的不育系及恢复系材料已选育至T_5。
A carrier constructed with sterile gene Barnase and its antagonist gene Barstar was transferred into cotton genome mediated by Agrobacterium. It was verified through molecular detection that 120 positive male sterile lines of cotton plants of T0 generation and 76 positive restorer lines of cotton plants of T_0 generation were got. By backcrossing, 5 male sterile lines of cotton of different traits and 3 restorer lines of cotton with good ability of recovery were bred.
出处
《山西农业科学》
2017年第7期1056-1057,1083,共3页
Journal of Shanxi Agricultural Sciences
基金
国家重点研发计划项目(2016YFD0101416)
关键词
棉花
杂种优势
雄性不育系
恢复系
cotton
heterosis
male sterile line
restorer line
