摘要
目的首次运用磁微粒化学发光免疫分析法探讨血清Dickkopf-1(DKK-1)检测在肝细胞癌(HCC)诊断中的临床应用价值。方法采用双抗体夹心磁微粒化学发光免疫分析法定量测定205例HCC患者、40例肝硬化患者和200例健康体检者血清DKK-1和AFP水平,计算ROC曲线下面积及DKK-1和甲胎蛋白(AFP)对HCC诊断的灵敏度和特异度。结果 HCC组DKK-1水平显著高于肝硬化组和健康体检组(P<0.01)。DKK-1对AFP阴性的HCC阳性率高达66.3%。ROC曲线结果表明:DKK-1最佳诊断界值为2.4ng/mL,AUC为0.822(95%CI:0.783~0.856),灵敏度为65.9%,特异度为87.5%。此外,DKK-1与AFP联合检测HCC曲线下面积(AUC)为0.915(95%CI:0.886~0.940),灵敏度为81.5%,高于任一单一指标检测(P<0.05)。结论 DKK-1对HCC的诊断具有重要价值,尤其对AFP阴性的HCC患者有较高的检出率,DKK-1联合AFP检测可显著提高HCC诊断的灵敏度及准确度。
Objective This is the first study to explore clinical application value of serum Dickkopf-1(DKK-1)detection in diagnosis of heptocellular carcinoma(HCC)by magnetic solid phase chemiluminescent immunoassay.Methods The level of serum DKK-1and AFP in 205 cases of HCC,40 cases of liver cirrhosis,and 200 cases of healthy control were quantitatively detected by Magnetic solid phase chemiluminescent immunoassay.The area under ROC curve,sensitivity and specificity of DKK-1and AFP for diagnosing HCC were calculated.Results The serum level of DKK-1 in HCC group was significantly higher than those of the liver cirrhosis group and healthy control group(P<0.01).DKK-1maintained diagnostic sensitivity for patients with HCC who were alpha-fetoprotein(AFP)negative(66.3%).ROC curves showed optimum diagnostic cut-off value was 2.4ng/mL,area under curve(AUC)was 0.822(95% CI:0.783-0.856),sensitivity 65.9%,and specificity 87.5%).Moreover,measurement of DKK1 and AFP together improved diagnostic accuracy for HCC versus all controls compared with either test alone [AUC 0.915,95%CI:0.886-0.940),sensitivity 81.5 %(P<0.05)].Conclusion Serum DKK-1detection has an important clinical value for diagnosis of HCC,especially for HCC with AFP negative.The combined detection of serum DKK-1and AFP can greatly increase sensitivity and accuracy for diagnosing HCC.
出处
《国际检验医学杂志》
CAS
2017年第13期1729-1731,共3页
International Journal of Laboratory Medicine
基金
国家自然科学基金资助项目(81371883)
