摘要
为了解贵州省小麦条锈菌群体的遗传多样性,本研究采用扩增片段长度多态性标记(AFLP)对其进行研究。结果显示:贵州省6个县市分离到的128个小麦条锈菌单孢子堆分离物中共获得32个AFLP基因型,没有检测到频率占绝对优势的AFLP基因型,其中1个AFLP基因型(zy6)频率(0.195)较高,AFLP基因型频率介于0.050~0.100的有3个,其余28个AFLP基因型的频率均低于0.050。香农系数及Φpt方法均显示亚群体间遗传差异不明显;Mantel test显示小麦条锈菌贵州群体遗传差异程度与地理距离无关;最大简约树步长评估结果显示小麦条锈菌贵州群体自由重组的可能性较低。表明,贵州省小麦条锈菌群体具有丰富的遗传多样性。
The population genetic diversity of wheat stripe rust（ Puccinia striiformis f. sp. Tritici） in Guizhou Province was analyzed by amplified fragment length polymorphism（ AFLP）. The results showed that32 AFLP genotypes were obtained from 128 isolates collected from 6 counties in Guizhou Province. No predominant genotype was identified. In which,1 genotype,zy6,had the highest frequency of 0. 195,3 genotypes had the frequency between 0. 050 ~ 0. 100,and the frequency of the other 28 genotypes was less than 0. 050.Shannon coefficient and Φpt statistics results showed that the genetic differentiation among 6 counties was not significant. Mantel test suggested that there was no significant correlation between genetic differentiation and geographical distances. The most parsimonious tree length results showed that the possibility of free recombination for population of Puccinia striiformis f. sp. tritici in Guizhou was very lower. The results indicated that the genetic diversity of P. striiformis was rich in Guizhou Province.
出处
《山东农业科学》
2017年第6期95-100,共6页
Shandong Agricultural Sciences
基金
国家自然科学基金项目(31301605
31371881)
天津市应用基础与前沿技术研究计划项目(15JCYBJC30800)
关键词
小麦条锈菌
群体遗传结构
AFLP标记
Puccinia striiformis f.sp.tritici
Population genetic structure
AFLP marker
