摘要
目的:观察萝卜硫素(SFN)对结直肠癌SW620细胞增殖、凋亡及迁移的影响,并探讨其可能的作用机制。方法:采用CCK-8法检测0、5、10、15、20μmol/L SFN处理48、72 h对SW620细胞增殖能力的影响,采用流式细胞术检测0、5、10、15、20μmol/L SFN处理24、48 h对SW620细胞凋亡的影响,采用细胞划痕实验观察20μmol/L SFN处理24 h对SW620细胞迁移的影响,并采用Western blot法检测细胞中STAT3、NF-κB、p-STAT3、p-NF-κB及MMP-2、MMP-9蛋白的表达。结果:随着SFN浓度的升高,SW620细胞增殖受到抑制,细胞凋亡率升高(P<0.05)。同时20μmol/L SFN可明显减弱SW620细胞的迁移能力,降低p-STAT3、p-NF-κB与MMP-2及MMP-9蛋白的表达(P<0.05)。结论:SFN抑制SW620细胞增殖、促进其凋亡的机制可能与降低p-STAT3、p-NF-κB的表达有关,抑制侵袭及转移的机制可能与其降低MMP-2及MMP-9蛋白的表达有关。
Aim： To observe the effects of sulforaphane（ SFN） on proliferation,apoptosis,and migration of SW620 cells in vitro and to explore the potential mechanism involved. Methods： SW620 cells were treated with 0,5,10,15,20μmol/L SFN for 48,72 h and the cell proliferation was detected by CCK-8 kit. SW620 cells were treated with 0,5,10,15,20 μmol/L SFN for 24,48 h and the cell apoptosis was detected by flow cytometry. SW620 cells were treated with 20μmol/L SFN for 24 h,the migration capability was measured by the wound healing test,and the protein expression levels of STAT3,p-STAT3,NF-κB,p-NF-κB,MMP-2 and MMP-9 were determined by Western blot. Results： SFN significantly reduced the proliferation and increased apoptosis in a dose-dependent manner（ P 〈 0. 05）. The ability of migration was significantly inhibited by 20 μmol/L SFN（ P 〈 0. 05）,and the expressions of p-STAT3,p-NF-κB,MMP-2,and MMP-9protein were significantly down-regulated（ P 〈 0. 05）. Conclusion： It is indicated that SFN could inhibit the proliferation and the ability of migration,and induce apoptosis of SW620 cells,which may be related with its down-regulating the expressions of p-STAT3,p-NF-κB,MMP-2 and MMP-9 protein.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2017年第3期281-284,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技厅基础与前沿技术研究基金112300410153
122300410155
