摘要
目的:建立气相色谱法测定灵芝孢子油中7个脂肪酸(肉豆蔻酸、十五烷酸、棕榈酸、十七烷酸、硬脂酸、油酸和亚油酸)含量的分析方法。方法:采用HP-FFAP(30 mm×0.32 mm×250μm)色谱柱,程序升温的方式对灵芝孢子油中7个脂肪酸含量进行测定研究。结果:各脂肪酸线性关系良好,重复性RSD均在3.0%以下,加样回收率(n=9)在96.6%~103.3%之间;样品测定结果显示在灵芝孢子油脂肪酸中,不饱和的脂肪酸约占总量的76.41%,以油酸和亚油酸为主,饱和脂肪酸以棕榈酸和硬脂酸为主,约占总量的18.05%。结论:该方法可作为检测灵芝孢子油中7个脂肪酸的推广方法。
Objective ： To develop a method for the determination of 7 fatty acids （ nutmeg acid, pentadecanoic acid, palmitic acid, heptadecanoic acid, stearic acid, oleic acid, linoleic acid ） in C, anoderma lucidurn spore oil using gas chromatography （ GC ）. Methods： The chromatographic column of HP-FFAP and the method of temperature programmed were adopted for quantitative analysis of 7 fatty acids in Ganoderma lucidum spore oil. Results： The linearity of 7 fatty acids in Ganoderma lucidum spore oil was good, RSD of repeatability was with less than 3.0%, and the average recoveries （ n=9 ） were 96. 6%-103.3%. The results showed that unsaturated fatty acids, rich with oleic acid and linoleic acid, accounted for about 76.41% of the total fatty acids in Ganoderma lucidum spore oil, saturated fatty acids, mainly as palmitic acid and stearic acid, accounted for about 18.05% of the total. Conclusion： The method has been successfully applied in determination of 7 fatty acids in Ganoderma lucidum spore oil.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2017年第6期982-987,共6页
Chinese Journal of Pharmaceutical Analysis
关键词
灵芝孢子油
脂肪酸
肉豆蔻酸
十五烷酸
棕榈酸
十七烷酸
硬脂酸
油酸
亚油酸
气相色谱法
Ganoderma lucidum spores oil
fatty acid
nutmeg acid
pentadecanoic acid
palmitic acid
heptadecanoic acid
stearic acid
oleic acid
linoleic acid
GC
