摘要
本实验以初始体质量为(4.88±0.90)g、体长为(4.85±0.32)cm的红小丑鱼(Amphiprion frenatus)为养殖对象,研究饲料中添加0(对照组)、2‰、4‰、6‰、8‰的虾青素对红小丑鱼消化酶活性的影响。本试验每组3个重复,每个重复6尾鱼,饱食投喂2次/d(投喂时间为08∶30和14∶30),试验周期为20 d。结果表明:添加虾青素组的红小丑鱼胃肠消化酶活性均高于未添加虾青素组,当虾青素添加量为4‰时,红小丑鱼前肠蛋白酶、脂肪酶、淀粉酶以及后肠淀粉酶活性达到最高(P<0.05);当添加量为6‰时,其胃脂肪酶和淀粉酶活性最高(P<0.05);当添加量为8‰时,其胃和后肠的蛋白酶以及后肠脂肪酶的活性达到最高(P<0.05)。因此,在饲料中添加4‰~8‰的虾青素能够较有效地提高红小丑鱼消化器官的消化酶活性。
Clown fish( Amphiprion frenatus) was one of the most popular marine ornamental fish,and its market share was increasing in aquaculture industry. Astaxanthin could improve the growth rate and immune response of fish,and digestive enzyme could affect the growth performance of fish. This study analyzed the effect of dietary astaxanthin on the digestive enzyme activity of clown fish. 90 juveniles of 30 days-post-hatch with the average weight and length of( 4. 88 ± 0. 32) g and( 4. 85 ± 0. 32) cm were divided into 5groups,which was possessed of the same 3 replicates,and were fed with basal diet containing 0,2‰,4‰,6‰ and 8‰ astaxanthin for 20 days,respectively. The results indicated that dietary astaxanthin increased the digestive enzyme activities of clown fish. When dietary astaxanthin content was 4‰,the activities of anterior intestine protease,lipase,amylase and posterior intestine amylase were highest( P < 0. 05). When dietary astaxanthin content was 6‰,the activities of stomach lipase and amylase were highest( P < 0. 05).When dietary astaxanthin content was 8‰,the activities of posterior intestine protease,amylase and pepsase were highest( P < 0. 05). In conclusion,the digestive enzyme activities of A. frenatus could be effectively improved through feeding basal diet with 4‰ ~ 8‰ astaxanthin.
出处
《渔业研究》
2017年第3期188-194,共7页
Journal of Fisheries Research
基金
基金项目:小丑鱼早期性腺发育分化的研究(2016R1101005-1)
