摘要
本研究将仿生学方法制备的纳米晶矿化胶原(nHAC)与聚乳酸(PLA)复合制备原材料,用于熔融挤压堆积成型(FDM)法制备骨修复体。采用碱性磷酸酶(ALP)试剂盒检测细胞ALP活性,扫描电镜观察细胞与材料间的相互作用,并观察到细胞出现黏附增殖。在动物实验中,植入个性化的仿生骨修复体,结果显示复合骨修复体逐渐降解并被新长出的骨组织所取代。实验表明了3D打印的骨修复体及制备的nHAC/PLA复合线材降解性能好,具备良好的细胞黏附和细胞相容性且细胞毒性较小,并能促进成骨细胞的增殖,从而促进骨愈合。
In this study,the nanocrystalline mineralized collagen(nHAC)prepared by the biomimetic method was combined with polylactic acid(PLA)to prepare the raw material which was used to prepare bone repair materials with the fused deposition modeling(FDM)method.The alkaline phosphatase(ALP)kit was used to detect the ALP activity of the cells,the scanning electron microscope was used to observe the interaction between the cells and the materials,and the adhesion and proliferation of the cells were observed.In animal experiments,personalized bionic bone repair materials were implanted,and the results showed that the composite bone repair materials gradually degrade and can be replaced by newly grown bone tissue.Experiments have shown that the three-dimensional printed bone repair material and the prepared nHAC/PLA composite wire have good degradation performance,good cell adhesion and cytocompatibility,low cytotoxicity,and can promote the proliferation of osteoblasts,thereby promoting bone healing.
作者
胡堃
王坤兰
崔玉珠
肖永昊
杨桂娟
孙玮玮
王峻东
王海波
郭林鑫峥
韩璐
李路海
莫黎昕
杨永刚
邓普君
马天一
危岩
HU Kun;WANG Kun-lan;CUI Yu-zhu;XIAO Yong-hao;YANG Gui-juan;SUN Wei-wei;WANG Jun-dong;WANG Hai-bo;GUO Lin-xinzheng;HAN Lu;LI Lu-hai;MO Li-xin;YANG Yong-gang;DENG Pu-jun;MA Tian-yi;WEI Yen(School of Printing and Packaging Engineering,Beijing Institute of Graphic Communication,Beijing 102600,China;Beijing Engineering Research Center of Printed Electronics,Beijing Institute of Graphic Communication,Beijing 102600,China;Bioprinting Engineering Technology Center,Beijing Institute of Graphic Communication,Beijing 102600,China;College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108,China;Department of Chemistry,Tsinghua University,Beijing 100084,China;Frontier Polymer Research Center,Tsinghua University,Beijing 100084,China)
出处
《数字印刷》
CAS
北大核心
2021年第3期147-157,共11页
Digital Printing
基金
北京印刷学院校级项目--普通重点教学改革项目--基于OBE教育模式的大学生创新创业能力提升研究--《高分子科学进展II》课程为例(No.22150121033/006)
3D打印及在生物医学中的应用(No.11000300653)
3D打印定制化骨材料及模具设计制备(No.11000300880)
3D打印教育模型的制备(No.11000300897)
创新人才培养北京五中化学基础翱翔学员培养与课程开发(No.11000301026)。
