摘要
[目的]探讨黄条鰤(Seriola aureovittata)在急性低氧胁迫下的应激响应机制。[方法]将体质量(621.13±63.29)g的黄条鰤置于养殖水槽中,通过药物调节水体中溶解氧含量,使其控制在(2.0±0.2)mg/L内,分别急性低氧胁迫0(对照组)、2、4和6 h,分析其肝脏和肌肉中氧化应激指标的变化。[结果]急性低氧胁迫后,黄条鰤肝脏中总超氧化物歧化酶(T-SOD)活性降低,与胁迫前相比差异显著(P<0.05);过氧化氢酶(CAT)活性与胁迫前相比显著升高(P<0.05);总抗氧化能力(T-AOC)随着胁迫时间的变化呈现先下降后升高的趋势;酸性磷酸酶(ACP)和碱性磷酸酶(AKP)活性都呈现先升高后下降的趋势。急性低氧胁迫后,在黄条鰤肌肉中总抗氧化能力(T-AOC)显著降低(P<0.05),而总超氧化物歧化酶(T-SOD)活性则显著升高(P<0.05)。[结论]急性低氧胁迫会影响鱼体肝脏和肌肉中的抗氧化酶及磷酸酶的活性,在低氧胁迫过程中黄条鰤会通过调节抗氧化酶的活性来激活体内抗氧化防御系统,从而保护机体免受缺氧导致的氧化损伤。
[Objective]To explore the stress response mechanism of Seriola aureovittata under acute hypoxic stress.[Method]S.aureovittata with the body mass of(621.13±63.29)g was placed in the aquaculture tank,the content of dissolved oxygen in the water was adjusted by drugs.The content of dissolved oxygen in the water was controlled within the range of(2.0±0.2)mg/L for acute hypoxic stress for 0(control group),2,4,and 6 hours,the changes of oxidative stress indices in the liver and muscle of S.aureovittata were analyzed.[Result]After acute hypoxia stress,total superoxide dismutase(T-SOD)activity in the liver of S.aureovittata reduced,which was significantly different from that before the stress(P<0.05).Catalase(CAT)activity in S.aureovittata significantly increased after acute hypoxic stress(P<0.05).Total antioxidant capacity(T-AOC)showed a trend of first decline and then rise with the stress time.The activities of acid phosphatase(ACP)and alkaline phosphatase(AKP)showed an trend of first increase and then decrease.In the muscle of S.aureovittata,the total antioxidant capacity(T-AOC)significantly reduced after acute hypoxic stress(P<0.05),while the total superoxide dismutase(T-SOD)activity significantly increased(P<0.05).[Conclusion]Acute hypoxia stress would affect the antioxidant enzymes and phosphatase activities in the liver and muscle of S.aureovittata.During hypoxia stress process,S.aureovittata would activate the antioxidant defense in the body by regulating the activity of antioxidant enzymes,so as to protect the body from oxidative damage caused by hypoxia.
作者
曹新宇
高铭鸿
杨旭
杨祯
汪忠林
刘欣悦
姜晨
CAO Xin-yu;GAO Ming-hong;YANG Xu(Key Laboratory of Mariculture&Stock Enhancement in North China’s Sea,Ministry of Agriculture and Rural Affairs,Dalian Ocean University,Dalian,Liaoning 116023)
出处
《安徽农业科学》
CAS
2022年第3期88-91,共4页
Journal of Anhui Agricultural Sciences
基金
国家重点研发计划项目(2019YFD0901005)。
关键词
黄条鰤
低氧胁迫
抗氧化酶
磷酸酶
应激响应机制
Seriola aureovittata
Hypoxia stress
Antioxidant enzymes
Phosphatase
Stress response mechanism
