摘要
目的 探讨了上皮型钙黏蛋白（Cdh1）蛋白在食管癌中的表达水平及与临床病理特征及预后的关系。
方法 分析来我院接受食管癌手术的132例患者的临床资料，分别以132例患者的食管癌组织（观察组）和癌旁组织（对照组）作为研究对象，采用实时荧光定量聚合酶链反应（FQ-PCR）、Western blot和免疫组织化学分析两组患者组织中Cdh1 mRNA和蛋白表达水平，分析Cdh1蛋白表达水平与患者临床病例特征和预后的关系。
结果 与对照组比较，观察组患者Cdh1 mRNA和蛋白质水平明显下调，差异有统计学意义（P＝0.004，P＝0.009）。免疫组织化学显示Cdh1蛋白主要表达于食管组织的细胞膜上，对照组和观察组Cdh1蛋白的表达阳性率分别为100%和21.96%，差异有统计学意义（P＝0.021）。Cdh1蛋白的表达与患者的年龄、性别、民族和组织学分类无明显相关，而与患者的淋巴结转移、TNM分期和浸润程度明显相关（P＝0.012）。观察组患者术后Cdh1阳性组患者1年和3年累积生存率明显高于阴性组患者，差异有统计学意义。
结论 Cdh1蛋白在食管癌组织表达水平明显下降，与患者淋巴结转移、浸润程度、TNM分期和预后密切相关。
Objective To explore the cadherin 1 （Cdhl） protein expression level in esophageal cancer and its relationship with clinical pathological characteristics and prognosis. Methods Retrospectively analysis the clinical data of 132 patients of esophageal cancer surgery in our hospital. 132 cases e- sophageal cancer tissue （observation group） and tissue adjacent to carcinoma （control group） were select- ed as the research object. Cdhl mRNA and protein expression level in control group and observation group were analyzed by real - time fluorescent quantitative PCR, Western blotting and immnnohistochemistry. The relationship of Cdhl between clinical features and prognosis were analyzed. Results Compared with control group, Cdhl mRNA and protein levels in the observation group significantly reduced, the difference was statistically significant （ P = 0. 004, P = 0. 009 ）. Immunohistochemical analysis showed that Cdhl pro- tein is mainly expressed in cell membrane of esophageal tissue. Cdhl protein expression positive rate in the control group and observation group were 100% and 21.96%, respectively, the difference was statistically significant （P = 0. 021 ）. In esophageal cancer patients, the expression rate of Cdhl was correlated with the degree of differentiation of esophageal cancer, TMN stage, invasion depth and lymph node metastasis of e- sophageal cancer and was not correlated withpatient＇ s age, gender, nationality. In the observation group, the 1, 3 years of cumulative survival rate in Cdhl positive group significantly increased than that of negative group （P =0. 012）. Conclusion Cdhl protein expression level decreased obviously in esophageal canc- er, and the degree of the patients with lymph node metastasis, invasion, TNM stage and prognosis is closely related, can be used as auxiliary diagnostic marker of prognosis.
出处
《中华实验外科杂志》
CSCD
北大核心
2017年第4期678-680,共3页
Chinese Journal of Experimental Surgery
关键词
食管癌
上皮型钙黏蛋白
临床病理特征
预后
Esophageal cancer
Cadherin 1 protein
Clinical pathological features
Prognosis
