摘要
目的检测食管鳞状细胞癌中piRNA-19166的表达水平,分析其临床病理意义。方法应用实时荧光定量聚合酶链式反应(qRT-PCR)技术检测piRNA-19166在96例食管鳞状细胞癌组织和癌旁正常组织中的表达,并分析其与食管鳞状细胞癌患者病理资料的关系。应用qRT-PCR检测piRNA-19166在3种食管癌细胞和正常食管上皮细胞中的表达。结果 96例食管鳞状细胞癌组织中,71例piRNA-19166表达下降,25例表达正常或升高;与癌旁正常食管组织相比,piRNA-19166在食管癌细胞株EC9706、TE-1、ECa10中均呈低表达,其中ECa10细胞、EC9706细胞中piRNA-19166低表达差异有统计学意义(P <0.05或P <0.01)。本研究结果显示,piRNA-19166低表达与分化程度、淋巴结转移、TNM分期有显著相关性(P <0.01)。结论 PiRNA-19166可能是一种新的食管癌肿瘤抑制基因,低表达的piRNA-19166促进了食管癌的发生、发展和转移。
Objective To detect the expression of piRNA-19166 in esophageal squamous cell carcinoma and analyze its clinicopathological significance.Methods The expression of piRNA-19166 was detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR)in tissues of esophageal squamous carcinoma and their paracancerous normal esophageal tissues of 96 cases,and the relationship between the expression of piRNA-19166 and pathological materials was analyzed.The qRT-PCR was used to detect the expressions of piRNA-19166 in three types of esophageal cancer cells and normal esophageal epithelial cells.Results In the 96 cases of esophageal squamous cell carcinoma,the expression of piRNA-19166 was decreased in 71 cases and was normal or increased in 25 cases.Compared with the paracancerous normal esophageal tissues,the expressions of piRNA-19166 in esophageal cancer cell lines EC9706,TE-1 and ECa10 were relatively low,and the expressions of piRNA-19166 in ECa10 and EC9706 cells were significantly lower than those in the paracancerous normal esophageal tissues(P<0.05 or P<0.01).The results showed that the low expression of piRNA-19166 was significantly correlated with the degree of differentiation,lymph node metastasis and TNM staging(P<0.01).Conclusion The piRNA-19166 may be a new tumor suppressor gene in esophageal cancer,and the low expression of piRNA-19166 promotes the occurrence,development and metastasis of esophageal cancer.
作者
郭玉莲
颜云
王成海
GUO Yulian;YAN Yun;WANG Chenghai(Teaching and Research Section of Pathology,Medical College of Yangzhou University,Institute of Translational Medicine,Yangzhou,Jiangsu,225009;Department of Pathology,Baoying County People′s Hospital in Jiangsu Province,Baoying,Jiangsu,225800;Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases,Yangzhou,Jiangsu,225009)
出处
《实用临床医药杂志》
CAS
2020年第22期1-4,共4页
Journal of Clinical Medicine in Practice
基金
江苏省卫生健康委医学科研项目指导性项目(Z2019046)。
关键词
piRNA-19166
食管鳞状细胞癌
实时荧光定量聚合酶链式反应
piRNA-19166
esophageal squamous cell carcinoma
real-time fluorescence quantitative reverse transcription polymerase chain reaction
