摘要
目的建立稳定的移植性人结直肠癌动物模型。方法以BALB/c裸鼠为研究对象，按随机数字表法分为原位接种组和皮下接种组，分别经直肠原位（使用自制接种器）和右侧腋窝皮下接种0.1 ml细胞密度为2×107/ml的人结直肠癌HCT116细胞悬液，观察60 d，比较两种造模方法的成瘤情况及肿瘤生长情况。
结果直肠原位接种组18只裸鼠全部成瘤，成瘤率为100%，平均瘤质量为（2.78±1.86）g，平均存活时间为（45.00±11.99）d；皮下接种组18只裸鼠中5只成瘤，成瘤率为27.78%，平均瘤质量为（1.74±0.82）g，平均存活时间为（60.00±0.00）d。结论采用自制接种器将0.1 ml细胞密度为2×107/ml的人结肠癌细胞HCT116接种于BALB/c裸鼠直肠原位可成功建立原位移植性人结直肠癌动物模型。
ObjectiveTo establish a stable animal model of transplanted human colorectal cancer.MethodsBALB/c nude mice were randomly divided into orthotopic colorectal model group and subcutaneous inoculation model group by random number table, and separately inoculated with 0.1 ml human colon cancer cell HCT116 under the density of 2×107/ml into the orthotopic colorectal （with the self-made inoculator） and right forelimb pit subcutaneously. The mice were observed for 60 days to compare the tumor formation and tumor growth in the two groups.ResultsThe tumor formation rate of all 18 animals was 100% （18/18） in the orthotopic colorectal group. The average tumor weight was （2.78±1.86） g and the average survival time was （45.00±11.99） d. The tumor formation rate was 27.78% （5/18） in the subcutaneous inoculation group. The average tumor weight was （1.74±0.82） g, and the average survival time was （60.00±0.00） d.Conclusion0.1 ml （2×107/ml） human colon cancer cell suspension HCT116 inoculated into BALB/c nude mice orthotopic colorectal with self-made inoculator could establish human colorectal cancer animal model successfully.
出处
《肿瘤研究与临床》
CAS
2017年第4期223-226,共4页
Cancer Research and Clinic
