摘要
目的比较单一情绪刺激与复合情绪刺激对肝郁证动物模型的影响,以期筛选出一种更加贴近临床肝郁证候表现的造模方法。方法通过前期筛选,分别建立夹尾法,四肢捆绑法和颈部带枷法的单一造模模型和三种方法复合造模的模型。建立肝郁证人与大鼠证候对照表以及肝郁证大鼠证候赋分表,结合中医证候指标、一般观察、体重指标、阳性药物反证以及体内激素指标的测定,以验证造模结果。结果正常组大鼠生长状况良好;模型组大鼠主要表现为急性应激躁狂和慢性应激抑郁,粪便干、少、小,多数出现眼睛暗红、脱毛、呼吸急促的症状,大鼠体重增长率降低,下丘脑CRH水平、血浆CORT、ACTH水平与正常对照组相比显著升高,且复合造模组成模效果效果明显优于单一造模组;阳性药物组经药物治疗后其症状明显好转,各指标也趋近正常组。结论使用夹尾、四肢捆绑和颈部带枷复合情志造模法构建肝郁证大鼠模型周期短、稳定性好、成功率高,模型动物表现与临床肝郁证证候有较高的一致性。
Objective To compare the effects of single and complex emotional stimuli on animal model of liver depression syndrome, in order to find out an animal model of liver depression syndrome with more similar clinical manifestations. Methods Based on the previous screening work, different methods, i.e. tail clamping, limb binding, putting a cangue on the neck, were used respectively, or using the three methods in combination to establish rat models of liver depression syndrome. A comparison table of human and rat liver depression syndrome was prepared, a mark sheet of evaluation criteria was made, general condition was observed and body weight was measured, and positive response to drugs and changes of hormonal levels were evaluated to identify whether the models were established successfully. Results Rats in the control group grew well and showed no abnormalities. There were lots of abnormal phenomena in the model group especially in the complex emotional stimulus modeling group. They were maniac with acute stress or depressive with chronic stress, had dry hair, small and less feces, many of the rats had hair loss, red eyes and shortness of breath symptom. They showed a significantly lower body weight gain and higher levels of CRH in hypothalamus and plasma ACTH and CORT. The manifestations were much more severe in the complex-emotional stimuli-induced model group than that in the singlestimulus prepared model groups. The drug-treated group showed considerable alleviation of the symptoms and improvement of the parameters closing to normal levels. Couelutious Establishing the rat model of stagnation of liver depression syndrome by complex emotional stimuli modeling method has good stability, with a short preparation period and high success rate, and presenting a better consistence with clinical liver stagnation of depression syndrome, so as to provide a suitable tool for TCM research.
出处
《中国比较医学杂志》
CAS
北大核心
2017年第4期82-88,共7页
Chinese Journal of Comparative Medicine
基金
国家重大新药创制重大专项课题:中药复方药理学研究与药效评价关键技术(2009ZX09502-015)
山东省自主创新和成果转化项目(2014ZZCX02104)
国家自然科学基金面上项目(81374059)
泰山学者工程专项经费资助(Ns201511107)
关键词
情绪刺激
肝郁证
大鼠模型
Emotional stimulus
Liver stagnation
Depression syndrome
Rat model
