摘要
目的 建立中医肝郁证大鼠模型。方法 利用不可预知的多种应激随机组合方法 ,观察大鼠体重及对 1%蔗糖水的摄取量 ,放免法测定血浆促皮质素 (ACTH)水平。结果 模型组大鼠在第2周末和第 3周末的 1%蔗糖水摄取量与实验前相比明显减少 (P <0 0 5 ,P <0 0 1) ;阿米替林组大鼠摄取量在实验的 3周内未见差异变化 ;逍遥散组大鼠摄取量则在第 2、3周末时与实验前相比增加 (P <0 0 5 )。在实验的第 2、3周末 ,模型组大鼠 1%蔗糖水摄取量明显少于对照组 ,差异有显著性意义 (P <0 0 1,P <0 0 5 ) ,而逍遥散组大鼠则明显多于对照组 ,差异有显著性意义 (P <0 0 5 ,P<0 0 1) ,阿米替林组与对照组比较未见显著性差异。与对照组比较 ,模型组、阿米替林组和逍遥散组大鼠体重在实验的第 1、2、3周末时均明显低于对照组 (P <0 0 5 ,P <0 0 0 1) ,但在第 2、3周末时 ,阿米替林组和逍遥散组大鼠体重显著高于模型组 (P <0 0 5 ,P <0 0 1)。模型组大鼠血浆ACTH水平升高 (P <0 0 1) ,逍遥散组和阿米替林组可使ACTH的升高部分地恢复 (P <0 0 1,P <0 0 5 )。结论 利用慢性不可预知的多种应激随机组合的方法 ,通过观测 1%蔗糖水摄取量的变化 ,可较好地模拟中医肝郁证的核心表现 。
Objective To establish the rat model of liver depression syndrome. Methods: A method of random combinations of various kinds of unpredictable chronic stress was adopted in the establishment of the model of liver depression syndrome in rats; during the experiment, the body weight of the rats and the consumption of 1% sugar water by the rats were observed, and the level of ACTH in the plasma of the rats was detected by radioimmunoassay. Results The consumption of 1% sugar water markedly lowered at the ends of the 2nd and the 3rd weeks of the experiment, respectively, in the rats in the model group, as compared with that before the experiment ( P<0 05, P<0 01 ); the consumption did not show notable change during all the 3 weeks of experiment in the amitriptyline group; the consumption increased at the ends of the 2nd and the 3rd weeks in the Xiaoyao Powder group, as compared with that before the experiment ( P <0 05); the consumption was significantly less at the ends of the 2nd and the 3rd weeks in the model group than that in the control group ( P<0 01, P<0 05 ); the consumption was significantly bigger at the ends of the 2nd and the 3rd weeks in the Xiaoyao Powder group than that in the control group ( P<0 05, P<0 01 ); and there was no significant difference between the consumption in the amitriptyline group and that in the control group. As compared with the body weight of the rats in the control group, that of the rats in the model group, amitriptyline group and Xiaoyao Powder group all markedly lowered at the ends of the 1st, 2nd, and 3rd weeks (P<0 05, P<0 001 ) while the body weight of the rats in the amitriptyline group and Xiaoyao Powder group was markedly higher at the ends of the 2nd and the 3rd weeks than that of the rats in the model group ( P<0 05, P<0 01 ). The level of ACTH increased in the plasma of the rats in the model group ( P <0 01) while the increased level of ACTH decreased to a certain degree in the amitriptyline group and Xiaoyao Powder group ( P<0 01, P<0
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2003年第2期18-21,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金课题 (No.3 0 17112 9)
北京中医药大学"2 11工程"资助课题
关键词
肝郁证
慢性应激
1%蔗糖水摄取量
逍遥散
大鼠
Liver Depression Syndrome
Chronic Stress
Consumption of 1% Sugar Water
Xiaoyao Powder
Rat
