摘要
通过建立^(60)Co γ辐射损伤小鼠模型,探究雅安藏茶茶褐素对^(60)Co γ辐射损伤的防护作用。实验选取48只雄性SPF级小鼠,随机分成正常对照组、辐射对照组、阳性对照组,以及雅安藏茶茶褐素低、中、高三个剂量组,连续灌胃相应受试样品15 d,其中在灌胃第6 d,除正常对照组外,其余各组均进行全身一次性剂量为5 Gy的^(60)Co γ射线照射。第16 d称量体重后,小鼠眼睑取血、解剖后,测定其外周血细胞、骨髓脱氧核糖核酸(Deoxyribonucleic acid,DNA)含量、胸脾指数、肝脏组织总抗氧化能力等相关指标。结果表明,与辐射对照组相比,雅安藏茶茶褐素能极显著提高辐射损伤小鼠血液的白细胞(White Blood Cell,WBC)、血小板(Platelets,PLT)、淋巴细胞(Lymphocytes,LYM)数量以及血液超氧化物歧化酶(Superoxide Dismutase,SOD)、过氧化氢酶(Catalase,CAT)活性(p<0.01),极显著增强小鼠肝脏组织的总抗氧化能力(Total Antioxidant Capacity,T-AOC)、总超氧化物歧化酶(Total Superoxide Dismutase,T-SOD)活性(p<0.01),并使股骨骨髓DNA含量极显著升高(p<0.01);同时,使肝脏丙二醛(Malondialdehyde,MDA)含量极显著降低(p<0.01),明显缓解了小鼠免疫器官胸脾的萎缩。其效果随茶褐素浓度而提高,其中高剂量茶褐素的效果达到或优于阳性药物的效果。结果表明,茶褐素对^(60)Co γ照射的辐射损伤小鼠的抗氧化系统和造血系统有较好的防护作用,以高剂量的效果最好。
Background： Ya＇an Tibetan tea is one of the earliest dark teas in China. It has been drunk for more than a thousand years in the border, and has become a life tea and the main prescription for disease prevention of the ethnic minority. Purpose： The aim is to investigate the protective effect of the theabrownines （TB） of Ya＇an Tibetan tea on the damage of 60Co γ-ray radiation by establishing injury mice model caused by 60Co γ radiation. Methods： 48 male SPF mice were randomly divided into normal control group, radiation model group, and positive control group, low, medium and high-dose groups of the TB of Ya＇an Tibetan tea. The mice were continuously fed for 15 d. On the 6th day of intragastric administration, all of the mice except for the normal group were subjected to 60Co γ-ray irradiation with a dose of 5 Gy. On the 16th day after weighing, the mice were taken for blood from eyeballs and anatomy. The related indicators of the number of peripheral blood cells, deoxyribonucleic acid （DNA） content of bone marrow, chest spleen index, and total antioxidant capacity of liver tissues were determined. Results： Compared with radiation control group, the TB of Ya＇an Tibetan tea could significantly improve the number of white blood cells, platelets and lymphocytes in the blood of radiation-injured mice, and significantly enhance cell activity of blood superoxide dismutase and catalase （p〈0.01）, and could significantly increase total antioxidant capacity （T-AOC）, total superoxide dismutase （T-SOD） activity of liver tissue of the mice （p〈0.01） and bone marrow DNA content of the femur. At the same time, the content of malondialdehyde （MDA） in liver was significantly decreased （p〈0.01）. TB could significantly relieve the atrophy of thymus and spleen gland of the immune organs of the mice, and the effect increases with the increase of TB concentration. The effect of high dose group is comparable to or better than the effect of positive drug. Conclusion： The TB of Ya�
出处
《核技术》
CAS
CSCD
北大核心
2017年第4期7-14,共8页
Nuclear Techniques
基金
雅安市市校合作项目
雅安藏茶保健功能研究及成果应用示范推广(No.2016/09-2018/09)
四川省教育厅青年基金项目
四川边销茶的抗辐射作用研究(No.12ZA125)资助~~
