摘要
为了更好地开发利用西藏珍稀大型真菌亚东木耳,对亚东木耳菌丝生长和次级代谢条件进行研究。考察了不同培养基成分和培养条件对亚东木耳菌丝生长速度、生物量和次级代谢产物产量的影响。结果表明,可溶性淀粉、酵母浸粉和CaCl_2分别为亚东木耳生长代谢的较佳碳源、氮源和无机盐,维生素B_1、B_2对菌丝生长无明显促进作用。较佳培养温度为26℃,较佳培养时间为63 d,菌丝生长较佳初始pH值为7.0,较佳次级代谢初始pH值为6.0。HPLC图谱分析结果表明,次级代谢产物种类多达200余种,其中初始pH值和温度条件对次级代谢多样性的影响较为明显。
In order to utilize and develop the rare Yadong edible fungus（Exidia sp. ） better, the growth and metabolism culture conditions of the mycelium were explored. Effects of the culture mediums and the different conditions on mycelium growth rate, biomass, and secondary metabolites production were inves-tigated. The results showed that glucose was the most suitable carbon source for mycelial growth, and soluble starch was the most suitable carbon source to obtain maximum biomass and metabolic product yield. Yeast extract was the most suitable nitrogen source for growth and metabolism. CaCl2 was the opti-mal inorganic salt while vitamin B1 and B2 had no significant effects on promoting mycelial growth. HPLC fingerprint results showed that compounds of secondary metabolites were as many as 200 kinds and initial pH and temperature factors had more obvious influences on the secondary metabolic diversity.
出处
《食品科学技术学报》
CAS
2017年第2期51-57,共7页
Journal of Food Science and Technology
基金
2014年西藏自治区科技计划应用基础研究项目"高原地区双孢菇栽培技术研究与菌丝发酵及次生代谢活性分析"
关键词
大型真菌
亚东木耳
培养条件
次级代谢
HPLC分析
edible fungus
Yadong edible fungus
culture conditions
secondary metabolism
HPLC analysis
