摘要
采用基于核磁共振波谱技术的代谢组学技术,探索菲律宾蛤仔在受到鳗弧菌和灿烂弧菌感染的代谢物变化特征,构建菲律宾蛤仔对弧菌感染后的代谢网络调控图谱,并比较两种弧菌毒性效应的差异。试验结果表明,3种代谢物葡萄糖、谷氨酸、苏氨酸在两种弧菌感染时均发生了变化,表征鳗弧菌感染的代谢物为牛磺酸、精氨酸、酪氨酸、苯丙氨酸、赖氨酸、亮氨酸、异亮氨酸、缬氨酸;而表征灿烂弧菌污染的代谢物为甜菜碱、二甲基甘氨酸、胆碱、谷氨酸、亚牛磺酸。
In this work,we characterized the metabolic responses induced by Vibrio anguillarum and V.splendidus challenges towards hepatopancreas of Manila clam Ruditapes philippinarum using nuclear magnetic resonance（NMR）-based metabolomics.Metabolic responses showed that both V.anguillarum and V.splendidus induced disturbances in energy metabolism,osmotic regulation,protein biosynthesis and oxidative stress with different mechanisms.Three metabolites（glucose,glutamate and threonine）were altered in both V.anguillarumand V.splendidus exposed groups.The metabolic biomarkers in V.anguillarum-treated groups included leucine,isoleucine,valine,arginine,lysine,taurine,tyrosine,and phenylalanine.And the metabolic biomarkers in V.splendidus-treated groups included hypotaurine,dimethylglycine,choline,betaine,and glycine.The findings indicate that metabolomics could be used to elucidate the biological effects of marine pathogens to the clam.
作者
刘小莉
侯承宗
来涌凯
高军
李洁
尚秋爽
龙静
邓小玉
邱文彬
孙虎山
LIU Xiaoli HOU Chengzong LAI Yongkai GAO Jun LI Jie SHANG Qiushuang LONG Jing DENG Xiaoyu QIU Wenbin SUN Hushan(School of Life Sciences, Ludong University, Yantai 264025, Chin)
出处
《水产科学》
CAS
CSCD
北大核心
2017年第2期143-147,共5页
Fisheries Science
基金
国家自然科学基金资助项目(41506190)
山东省高等学校科技计划项目(J14LE08)
鲁东大学基金资助项目(LY2014018)
关键词
鳗弧菌
灿烂弧菌
菲律宾蛤仔
代谢物
核磁共振波谱技术
Vibrio anguillarum
Vibrio splendidus
Ruditapes philippinarum
nuclear magnetic reso nance（NMR）
metabolites
