摘要
应用流式细胞仪技术,通过检测血细胞的吞噬活力、活性氧产物和死亡率,研究了Cd2+和苯并芘(Bap)胁迫7d对文蛤血细胞功能的影响。结果表明,10μg/L和100μg/LCd2+胁迫会显著降低血细胞的吞噬活力,增加血细胞的死亡率,100μg/LCd2+会显著增加血细胞活性氧产物。5μg/L和50μg/LBap胁迫会显著降低文蛤血细胞的吞噬活力,增加血细胞的死亡率,50μg/LBap会显著增加血细胞活性氧产物。可见,Cd2+和苯并芘胁迫会显著降低文蛤血细胞的功能,导致文蛤免疫力下降,增大文蛤被病害感染的机率。
The effects of cadmium and benzo[α]pyrene（Bap） exposure on the immune function of Meretrix meretrix hemocytes were examined by flow cytometery.The results showed that 10 and 100 μg/L Cd^2＋ significantly suppressed phagocytosis of hemocytes and increased the mortality of hemocytes,while 100 μg/L Cd^2＋ stimulated reactive oxygen species（ROS） in hemocytes.Bap（5 and 50 μg/L） inhibited phagocytosis and increased the mortality of hemocytes,while 50 μg/L Bap could enhance ROS.The results suggested that cadmium and benzo[α]pyrene exposure significantly inhibited the immune function of hemocytes,which may lead to low immunity and increased infection of M.meretrix.
出处
《海洋科学》
CAS
CSCD
北大核心
2010年第9期82-86,共5页
Marine Sciences
基金
国家基金委创新研究群体科学基金项目(40821004)
国家973计划资助项目(2007CB407305)
国家农业部公益性计划资助项目(nyhyzx07-047)
国家海洋公益性行业科研专项资助项目(200805069)
