摘要
以黔产接骨草药材为研究对象,建立了福林酚比色法检测接骨草中总多酚含量方法;并采用单因素结合响应面优化设计法优化接骨草中总多酚提取条件;在此基础上,同时测定接骨草不同产地、不同药用部位总多酚的含量,并以清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基和ABTS自由基的能力为评价指标,对接骨草不同药用部位抗氧化活性进行比较研究。结果表明,不同产地、不同药用部位接骨草中总多酚含量差异较大;同批次接骨草中总多酚含量依次为叶>全草>茎>根;接骨草中叶、全草、茎和根清除DPPH的IC50值分别为1.14、1.70、2.25、2.65 mg/m L,清除ABTS的IC50值分别为1.16、1.56、2.17、3.15 mg/m L,且活性大小与总多酚质量浓度呈现明显量效关系。该方法简便、快捷、重复性好,为接骨草基于总多酚含量的质量评价和质量控制提供参考,以IC50作为判定抗氧化活性强弱的指标,4个不同药用部位中以接骨草叶提取物抗氧化能力相对较强。
In this study,a method was developed for the determination of total polyphenol by Folin-Ciocalteu colorimetry in Sambucus chinensis Lindl which was collected in Guizhou province. The single factor trials and response surface methodology（ RSM） were used to optimize the extraction process of polyphenols from S. chinensis. The developed method was applied for the determination of total polyphenol from different origins and parts of S. chinensis. Besides,the antioxidant activities of S. chinensis extract from different parts were investigated using in vitro antioxidant assays. The results showed that the content of total polyphenol of S. chinensis from different parts and different origins was different. The content in leaf 〉whole herb 〉stem〉 root. The IC50 of scavenging DPPH of leaves,whole herb,stems and roots were 1. 14,1. 70,2. 25,2. 65 mg/mL and ABTS radical 1. 16,1. 56,2. 17,3. 15 mg/mL,respectively. The method was simple,rapid and reproducible,which can be used for evaluating the quality and quantitative analysis of total polyphenols in S. chinensis.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第2期255-263,共9页
Natural Product Research and Development
基金
贵州省高层次创新人才培养项目[黔科合人才(2015)4033号]
贵州省科技厅贵州师范大学联合基金(黔科合LH字[2015]7768号)
贵州省中药材
民族药材质量标准研究项目
贵州省中药现代化科技产业研究开发专项(黔科合中药字[2013]5001号)
贵阳市科技计划(贵阳市科技重大专项)筑科合同[2012401]社-4号
关键词
接骨草
总多酚
含量测定
抗氧化活性
Sambucus chinensis Lindl
total polyphenols
content determination
antioxidant activity
