摘要
目的:探讨上皮–间质转化(epithelial-mesenchymal transition,EMT)在蒿甲醚(artemether,ARE)对同期放化疗抵抗人结直肠癌细胞HCT116(HCT116CRR)的逆转作用中的作用。方法:实验分为四组:1)对照组;2)单纯放化疗组;3)ARE联合放化疗组;4)ARE组。采用Real-time PCR和Western blot,检测各组E MT相关指标E-cad her in,N-cad her in,v iment in,snail mRNA及其蛋白的表达情况。结果:Real-time PCR和Western blot结果显示E-cadherin mRNA及其蛋白的表达情况,单纯放化疗组<ARE联合放化疗组<对照组<ARE组;N-cadherin,vimentin,snail mRNA及其蛋白的表达情况,单纯放化疗组>ARE联合放化疗组>对照组>ARE组。结论:EMT在ARE逆转同期放化疗抵抗人结直肠癌细胞HCT116CRR细胞系的放化疗抵抗作用中有重要的作用,即上调E-cadherin mRNA及其蛋白的表达,下调N-cadherin,vimentin,snail mRNA及其蛋白的表达。
Objective： To investigate the effect of epithelial-mesenchymal transition （EMT） on the reversion effect of chemo-radiation resistance of artemether （ARE） in human colorectal cancer HCT116CRR cell line. Methods： The experiment was divided into four groups： 1） Normal control group; 2） Chemoradiotherapy group; 3） ARE combined with chemoradiotherapy group; 4） ARE group. RT-PCR and Western blot was used to detect the expressions of mRNAs and proteins of E-Cadherin, N-Cadherin, Vimentin, Snail. Results： RT-PCR and Western blot results： E-cadherin mRNA and protein expression level, chemoradiotherapy group 〈 ARE combined with chemoradiotherapy group 〈 Normal control group 〈 ARE group （P〈0.05）; N-cadherin, Snail, Vimentin mRNAs and proteins expression level, chemoradiotherapy group 〉 ARE combined with chemoradiotherapy group 〉 Normal control group 〉 Artemether group （P〈0.05）. Conclusion： ARE plays a role of reversion to chemo-radiation resistance in human colorectal cancer HCT116CRR cell line by modulating the EMT-related molecular markers. That is to say, artemether can up-regulate the mRNA and protein of E-cadherin and down-regulate the mRNAs and proteins of N-cadherin, vimentin, and snail.
作者
王艳俊
蒋永新
刘珊
付凤莲
王红
WANG Yanjun JIANG Yongxin LIU Shan FU Fenglian WANG Hong(Key Laboratory of Lung Cancer in Yunnan Province Tumor Clinical Research Center Combined Traditional Chinese and Western Medicine, Third Affiliated Hospital of Kunming Medical University, Kunming 650118, China)
出处
《临床与病理杂志》
2017年第2期319-325,共7页
Journal of Clinical and Pathological Research
基金
云南省卫生厅项目(2014NS037)
云南省教育厅基础研究项目(2016ZDX061)
昆明医科大学硕士研究生创新基金项目(2016S35)~~
关键词
蒿甲醚
结直肠癌
肿瘤细胞
放化疗抵抗
上皮-间质转化
artemether
colorectal cancer
neoplasm cells
chemo-radiation resistance
epithelial-mesenchymal transition
