摘要
目的:研究蒿甲醚对胃癌细胞(AGS和SGC-7901)的增殖、侵袭以及迁移的影响并探讨其分子机制。方法:分别利用MTT、集落形成试验检测胃癌细胞的活力和生长;流式细胞技术检测胃癌细胞的凋亡率;利用细胞侵袭和划痕愈合试验检测胃癌细胞的侵袭和迁移;Western blot检测相关蛋白的表达水平。结果:蒿甲醚(0~800μmol/L)能够浓度和时间依赖性的抑制AGS和SGC-7901细胞的增殖(P<0.05)。集落形成试验以及流式细胞术结果显示蒿甲醚(400和600μmol/L)能够抑制AGS和SGC-7901细胞的生长以及促进细胞凋亡(P<0.05)。细胞侵袭和划痕愈合试验发现蒿甲醚(400和600μmol/L)能够抑制AGS和SGC-7901细胞的侵袭和迁移(P<0.05)。Western blot结果显示蒿甲醚(400和600μmol/L)能够增加AGS和SGC-7901细胞的cleaved caspase-3,cleaved caspase-9以及Bax的蛋白水平(P<0.05);同时能够抑制N-cadherin和vimentin的蛋白表达并促进E-cadherin蛋白的表达。结论:蒿甲醚可以显著抑制胃癌细胞的增殖、侵袭以及迁移,其机制可能是与促进细胞凋亡以及抑制上皮间质转化有关。
Objective:To investigate the effects of artemether on gastric cancer cell proliferation,invasion and migration and to explore the underlying mechanisms.Methods:MTT and colony formation assays were used to determine gastric cancer cell viability and cell growth,respectively.Flow cytometry evaluated the cell apoptosis.Transwell invasion and wound healing assays were used to determine gastric cancer cell invasion and migration,respectively.Western blot assay was used to determine protein expression levels.Results:Artemether(0~800μmol/L)suppressed cell viability of AGS and SGC-7901 cells in a concentration-and time-dependent manner(P<0.05).Colony formation assay and flow cytometry results showed that artemether(400 and 600μmol/L)suppressed cell growth and induced cell apoptosis of AGS and SGC-7901 cells(P<0.05).Transwell invasion and wound healing assays showed that artemether(400 and 600μmol/L)also inhibited cell invasion and migration of AGS and SGC-7901 cells(P<0.05).Western blot assay showed that artemether(400 and 600μmol/L)increased the protein levels of cleaved caspase-3,cleaved caspase-9 and Bax(P<0.05),and suppressed the protein levels of N-cadherin and vimentin,but increased E-cadherin protein level in AGS and SGC-7901 cells(P<0.05).Conclusion:Artemether exerted suppressive effects on gastric cancer cell proliferation,invasion and migration,which may be related to increased cell apoptosis and decreased epithelial mesenchymal transition.
作者
陈妮
赵梅
陈玲
韩鹏定
Chen Ni;Zhao Mei;Chen Ling;Han Pengding(Department of Pharmacy,the Second Affiliated Hospital of Hainan Medical University,Hainan Haikou 570311,China;Department of Pharmacy,the Sanya Central Hospital,Hainan Sanya 572000,China)
出处
《现代肿瘤医学》
CAS
2020年第10期1633-1638,共6页
Journal of Modern Oncology
基金
海南省卫生计生行业科研项目(编号:18A200124)
海南医学院科研培育基金项目(编号:HY2016-12)。
关键词
胃癌细胞
蒿甲醚
增殖
凋亡
侵袭和迁移
上皮间质转化
gastric cancer
artemether
proliferation
apoptosis
invasion and migration
epithelial mesenchymal transition
