摘要
目的探讨血清基质金属蛋白酶-9(MMP-9)、胱抑素C(cystatin c)、一氧化氮(NO)水平与脑梗死后患者认知功能障碍的关系。方法选择2015年3月至2016年7月收治的脑梗死后认知功能障碍患者100例为患者组,另选择同期收治的脑梗死患者无认知障碍者70例为对照组。采用酶联免疫吸附实验双抗体夹心法测定受试对象血清MMP-9,免疫比浊法测定受试对象血清cystatin c水平,硝酸还原酶比色法测定受试对象血清NO水平。同时分析蒙特利尔认知评估量表(MOCA)各项评分与血清MMP-9、cystatin c、NO水平的相关性。结果患者组的血清MMP-9含量为(462.7±64.2)ng/ml,明显高于对照组血清MMP-9含量(142.3±45.8)mg/L,差异有统计学意义(t=7.428,P<0.05);患者组血清cystatin c含量为(678.1±78.2)μg/L,明显高于对照组血清cystatin c含量(302.6±45.5)μg/L,差异有统计学意义(t=8.735,P<0.05);患者组血清NO含量为(121.4±15.6)mg/L,明显高于对照组血清NO含量(62.5±3.2)mg/L,差异有统计学意义(t=9.261,P<0.05)。Spearman相关分析显示,患者组血清MMP-9水平与MOCA总分呈负相关(r=-0.627,P<0.05);血清cystatin c水平与MOCA总分呈负相关(r=-0.605,P<0.05);血清NO水平与MOCA总分呈负相关(r=-0.526,P<0.05)。结论血清MMP-9、cystatin c、NO水平是诱发脑梗死后认知功能障碍的独立性危险因素,其和认知障碍严重程度密切相关,MMP-9、cystatin c、NO水平的高表达能够促进脑梗死后认知功能障碍的发生与发展。
Objective To investigate the relationship between serum matrix metalloproteinase-9（ MMP-9）,cystatin C and nitric oxide（ NO） levels and cognitive dysfunction after cerebral infarction. Methods From March 2015 to July 2016,100 patients with cognitive dysfunction after cerebral infarction were selected as the patients group,and 70 patients without cognitive impairment in the same period were selected as control group The level of serum cystatin c was determined by immuno-turbidimetric assay,and the serum NO level was determined by nitric acid reductase colorimetry. Meanwhile,the levels of serum NO were measured by enzyme-linked immunosorbent assay（ ELISA）（ MOCA） and serum MMP-9,cystatin C and NO levels in patients with chronic obstructive pulmonary disease（ AMI）. Results The level of serum MMP-9 in the patients was（ 462. 7 ± 64. 2） ng / ml,which was significantly higher than that in the control group（ 142. 3 ± 45. 8 mg /L）（ t=7. 093,P〈0.05）. The serum cystatin C level was（ 678. 1 ± 78. 2 μg / L）,which was significantly higher than that of the control group（ 302. 6 ± 45. 5） μg / L（ t=8. 126,P〈0.05）. The serum NO level in the patients was（ 121. 4 ± 15. 6 mg / L）,which was significantly higher than that in the control group（ 62. 5 ± 3. 2 mg / L）（ t=8. 405,P〈0.05）. Serum cystatin c level was negatively correlated with MOCA score（ r =-0. 627,P〈0.05）,and there was a negative correlation between serum MMP-9 level and MOCA total score in the patient group（ r =-0. 526,P〈0.05）. The serum level of NO was negatively correlated with the total score of MOCA（ r =-0. 526,P〈0.05）. Conclusion The serum levels of MMP-9,cystatin C and NO are independent risk factors for cognitive dysfunction after cerebral infarction,which are closely related to the severity of cognitive impairment. The high expression of MMP-9,cystatin C and NO can promote the occurrence and development of cognitive dysfunction after cerebral infarction.
出处
《临床和实验医学杂志》
2017年第5期452-454,共3页
Journal of Clinical and Experimental Medicine
