摘要
本研究旨在探讨鸡MOGAT和DGAT基因的表达和调控特性。首先克隆了具有MOGAT活性的MOGAT1、MOGAT2、LPGAT1和具有DGAT活性的DGAT2和SOAT1对应基因的编码区序列,并对其组织表达特性进行了研究;其次选取产蛋前期母鸡和产蛋高峰期母鸡作为试验材料,利用实时荧光定量PCR技术分析不同生理时期鸡肝中这些基因的表达变化规律;在此基础上利用雌激素处理的体内、体外试验,分析这些基因的表达调控机制。结果表明,鸡MOGAT1、MOGAT2、LPGAT1、DGAT2和SOAT1基因在各组织中表现出广泛表达的特性,并在脂类代谢旺盛的器官如肝、肾、小肠(十二指肠、空肠、回肠)等器官中表达较高,但DGAT2基因在鸡肝中的表达量较低,而MOGAT2基因主要在鸡小肠组织(十二指肠、空肠、回肠)中表达,MOGAT1在除胰腺外的大部分组织中都有表达;产蛋高峰期母鸡肝中MOGAT1、LPGAT1和SOAT1的表达量均显著低于产蛋前期母鸡;经不同浓度雌激素处理后,这些基因在鸡肝、十二指肠、肾以及鸡胚肝原代细胞中的表达量维持不变或显著下调。综上所述,已知的甘油三酯合成的单酰甘油通路中相关基因不是鸡肝TG合成代谢中的限速基因,在雌激素诱导的鸡TG合成代谢中,单酰甘油通路不发挥主导作用。
The objectives of the present study were to clone the genes in MOGATand DGATfamilies and characterize their expression and regulation properties in chicken.The coding sequences of genes including MOGAT1,MOGAT2,LPGAT1,DGAT2 and SOAT1which encoded proteins with enzymatic activities of MOGAT and DGAT families were cloned and sequenced.The tissue distribution of the genes was investigated by RT-PCR,and the expression patterns of genes in liver between pre-laying and peak-laying hens were detected by real-time PCR.In addition,the expression and regulation mechanism of the genes were explored using both in vivo and in vitro models treated with estrogen.The results showed that the genes in MOGATand DGATfamiliesexpressed extensively in chicken various tissues,and had relatively higher expression levels in the lipid metabolism organs such as liver,kidney,small intestine（duodenum,jejunum,ileum）,the DGAT2 gene had lower expression in liver and the MOGAT2 gene was mainly expressed in small intestine（duodenum,jejunum,ileum）.MOGAT1 was expressed in the most of tissues except pancreas.The expression levels of MOGAT1,LPGAT1 and SOAT1genes in liver of peak-laying hens were significantly lower than that in pre-laying hens.The expression levels of the genes in liver,duodenum,kidney and primary hepatocyte maintained no change or significantly decreased after the chickens and primary hepatocytes were treated with estrogen,respectively.In conclusion,the known genes in MOGATand DGATfamilies,which play important roles in mammalian TG metabolism,are not the key genes in TG synthesis in liver of chicken,and the monoacylglycerol pathway don＇t play a leading role in chicken TG synthesis metabolism induced by estrogen.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2017年第2期214-224,共11页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
河南省国际合作项目(162102410030)
国家自然科学基金(31372330)
农业部现代农业产业技术体系(CARS-41-K04)
河南省重大科技攻关计划项目(112101110800)
