摘要
以131头纯种南阳牛为研究材料,利用PCR-RFLPs对二酰甘油酰基转移酶2(DGAT2)基因的第6内含子和第7内含子的多态性及其与生长发育的相关性进行了分析。结果表明:南阳牛在该位点分别检测到两种等位基因A/B和N/M,频率分别为0.875/0.125和0.971/0.029。A和N等位基因是群体中的优势等位基因。该基因内含子6对南阳牛6月龄的体高、2岁体重、6月龄到两岁的胸围和体斜长都有显著的影响,内含子6的AA基因型的6月龄的体高比杂合型高3.8%,两岁体重高3.9%,6月龄到两岁的胸围分别高3.8%、3.4%、3.7%、4.3%;6月龄到两岁的体斜长分别高3.8%、3.6%、3.8%、3.1%。内含子7对18月龄和两岁的坐骨端宽有极显著的影响(P<0.01);对两岁的胸围有显著的影响(P<0.05)。
The associations between SNPs in intron 6 and intron 7 of the bovine diacylglycerolacyltransferase （DGAT2） gene and growth traits in purebred Nanyang cattle （n=131） were reported in this study. Alleles detected in intron 6 were allele A and B with frequencies of 0.875 and 0.125, respectively; alleles detected in intron 7 were allele N and M with frequencies of 0.968 and 0.032, respectively. The animals with genotype AA in intron 6 showed 3.8% increases in body height（P 〈 0.01）, 3.9% increases in body weight （P 〈 0.05）, 3.8% （P 〈 0.05）, 3.6% （P 〈 0.05）, 3.8% （P 〈 0.01） and 3.1%（ P 〈 0.01） increase in body length at age six-month to two-year compared with genotype AB, respectively. They also showed 3.8 % （P 〈 0.05）, 3.4 % （ P 〈 0.01）, 3.7% （P 〈 0.01） and 4.3% （P 〈 0.01） increases in heart girth at age six-month to two-year respectively. Animals with the NM genotype show significantly higher average heart girth at age two years （P 〈 0.05）, and Hucklebone width （P 〈 0.01） at age eighteen-month （P 〈 0.01） and two-year （P 〈 0.01） compared with animals with the genotype NN, respectively.
出处
《遗传》
CAS
CSCD
北大核心
2007年第8期945-950,共6页
Hereditas（Beijing)
基金
国家高技术研究发展计划项目(国家863计划)(编号:2006AA10Z197)
国家科技支撑计划项目(编号:2006BAD01A10-5)
河南省杰出人才创新基金(编号:0521001900)
西北农林科技大学拔尖人才支持计划项目资助~~
