摘要
目的研究γ射线对益母草种子的诱变效应,为采用益母草种子进行诱变育种提供参考依据。方法以益母草的干燥种子为材料,研究γ射线对其产生的生理效应和诱变效果。以种子发芽率、成苗率、幼苗苗高、根长,以及叶色突变率作为评价诱变效应的指标。结果γ射线对益母草种子发芽、幼苗生长有明显的抑制作用,具有较强的诱发叶色突变的能力。结论γ射线对益母草具有诱变作用,益母草干燥种子γ射线诱变育种的适宜辐射剂量范围为400~500 Gy。
OBJECTIVE To study the gamma-rays mutagenic effects on seeds of Leonurus japonicas Houtt., provide the basis for traditional Chinese medical herb breeding by using gamma-rays radiation. METHODS Physiological and mutagenic effects of gamma-rays on L. japonicas Houtt. dry seeds were studied. The germination percentage, seeding survival, seedling height, root length of M1 plants and the frequency of leaf color mutation in M2 generation were selected as criteria. RESULTS The gamma-rays showed obvious inhibitory action to the seedling growth and the ability to induce leaf color mutation. CONCLUSION The gamma-rays is one kind of L. japonicas Houtt. effective mutagens. The appropriate dose is 400-500 Gy for the dry seeds of L. japonicas Houtt.
作者
罗页思
徐建中
孙健
沈晓霞
王志安
LUO Yesi XU Jianzhong SUN Jian SHEN Xiaoxia WANG Zhian(Phamacy College of Zhejiang Chinese Medical University, Hangzhou 310053, China Zhejiang Research Institute of Traditional Chinese Medicine Co,, Ltd., Hangzhou 310023, China)
出处
《中国现代应用药学》
CAS
CSCD
2017年第1期12-15,共4页
Chinese Journal of Modern Applied Pharmacy
基金
浙江省农业新品种选育重大科技专项(2016C02058)
公益性行业科研专项经费项目(201507002)
关键词
60Co-γ射线
益母草
诱变育种
60Co-γ ray
Leonurus japonicas Houtt
mutation breeding
