摘要
目的:观察阿司匹林对人结肠癌肿瘤干细胞标志物CD44的影响,以探讨其对结肠癌干细胞的作用。方法:Western blot检测不同浓度阿司匹林作用后CD44在人结肠癌细胞株HT-29和SW480中的表达情况。结果:Western blot结果显示肿瘤干细胞标志物CD44在人结肠癌细胞株HT-29和SW480中均有表达,阿司匹林能降低HT-29和SW480中CD44蛋白的表达,并具有良好的量-效关系(P<0.05);两种细胞中CD44蛋白的表达比较差异均无统计学意义(P>0.05)。结论:阿司匹林能降低结肠癌细胞株HT-29和SW480中CD44的表达,并对结肠癌肿瘤干细胞有抑制作用。
Objective：To investigate the effect of Aspirin on the expression of cancer stem cell marker CD44 in human colon cancer.Method：Western blot was used to detect the expression of CD44 in human colon cancer cell line HT-29 and SW480 after different concentrations of Aspirin.Result：Western blot showed that cancer stem cell marker CD44 expression in both HT-29 and SW480 human colon cancer cell lines,Aspirin could reduce the expression of CD44 HT-29 and SW480,it had a good dose-response relationship（P〈0.05）.The expression of CD44 protein in two cells showed no significant difference（P〉0.05）.Conclusion：Aspirin can reduce the expression of CD44 in colon cancer cell line HT-29 and SW480,inhibit the proliferation of colon cancer stem cells.
出处
《中国医学创新》
CAS
2017年第1期1-4,共4页
Medical Innovation of China
基金
贵州省高层次人才科研条件特助经费资助项目(TZJF-2011-32)
贵州省科学技术基金项目(CK-988)
遵义医学院附属医院院资金项目(F-572)
