摘要
目的探讨抵抗素基因(RETN)的单核苷酸多态性(SNPs)位点rs2161490和rs1423096基因型与广东地区2型糖尿病(T2DM)的相关性。方法收集2015年1~11月广州医科大学附属第二医院收治广东地区的新诊断为2型糖尿病患者178例为病例组和体检中心患者192例为对照组,先分析两组的基因分布频率是否达到遗传平衡具有群体代表性,比较病例组与对照组抵抗素rs2161490和rs1423096两个基因位点出现的频率是否具有统计学意义,然后比较病例组和对照组血清抵抗素rs2161490 T→C基因型和rs1423096A→G基因型的分布频率,logistic回归分析:分析抵抗素rs2161490和rs1423096两个位点各个基因型对T2DM的危险度以及调整性别以及年龄两个变量后危险度的变化;比较病例组与对照组血脂生化指标;对病例组rs2161490和rs1423096位点各个基因型对应的血脂生化指标TG,CHOL,HDL-C和LDL-C的水平进行相关性分析。结果样本符合哈代-温伯格遗传定律,具有群体代表性,比较病例组与对照组抵抗素rs2161490和rs1423096两个基因位点频率:rs2161490的基因型(TT,CT,CC)比较,差异无统计学意义(P=0.834,>0.05);rs1423096的基因型(AA,AG,GG),相比较,差异无统计学意义(P为0.960,>0.05);各基因型与T2DM危险度分析,差异无统计学意义(P>0.05),调整性别以及年龄两个变量后危险度变化,差异无统计学意义(P>0.05);TG,CHOL,HDLC和LDL-C在各基因型表达浓度水平相关性分析,差异无统计学意义(P>0.05)。结论分析结果显示两位点各基因型在病例组与对照组的出现频率无统计学意义(均为P>0.05),rs2161490与rs1423096两位点各基因型与T2DM的危险度,调整前与协变量调整后均无统计学意义(均为P>0.05),rs2161490与rs1423096两位点各基因型与血脂水平分析差异同样均无统计学意义(均为P>0.05),从而推断两个基因型不是广东地区T2DM的患病风险基因。
Objective To explore the correlation between resistins rs2161490and rs1423096 genotype with type 2 diabetes mellitus （T2DM） in Guangdong. Methods Collected 178 blood of newly diagnosed T2DM in the Second Affiliated Hospital of Guangzhou Medical University from January 2015 to November 2015 as the patient group and 192 blood of healthy physi- cal examination as the control group. Analysis of the two groups of gene distribution frequency was to reach the genetic equi- librium, comparative two gene loci frequencies of resistin rs2161490 and rs1423096 in case group and control group was sta tistically significant,and compared the distribution frequency of rs2161490 locus T→C and rs1423096 locus A→G between the patient group and the control group. Then made a logistic regression analysis：analysing the risk two loci each genotype of resistin rs2161490 and rs1423096 to T2DM,adjust of the gender and age,and the changes of the risk of the two variables. Comparative blood lipids biochemical indexes between case group and the control group,mode the correlation analysis be tween TG,CHOL,HDL C and LDI.-C levels of serum lipids in patients with rs2161490 and rs1423096 each genotypewere performed. Results The sample was consistent with Weinberg Hardy＇s law of inheritance,which was representative of the population,comparing two gene loci frequency of resistin rs2161490 and rs1423096 of case group and control group： corn parinng CT, TT,CC of rs2161490 genotype, there was no statistically significant difference （ P = 0. 834, 〉 0.05 ）, and corn parinng AA,AG,GGof rs2161490 genotype,there was no statistically significant difference （P =0. 960,〉0. 05）. Each gen- otypes with T2DM risk analysis, there was no statistically significant difference（P〉0.05）. Adjusting the risk change afterthe two variables, gender and age, there was no statistically significant difference （P〉0.05） ; TG,CHOL, HDL-C and LDL- C in each of the genes expression levels correlation analysis, there was no statistically signific
出处
《现代检验医学杂志》
CAS
2017年第1期48-52,共5页
Journal of Modern Laboratory Medicine
基金
广东省医学科研基金项目资助(B2015040)
关键词
2型糖尿病
抵抗素
单核苷酸多态性
type 2 diabetes mellitus
resistin
single nucleotide polymorphism
