摘要
旨在研究雌激素与前列腺素受体对奶牛输卵管上皮细胞输卵管蛋白合成与分泌的调控作用。用荧光定量PCR检测了奶牛输卵管上皮细胞输卵管蛋白mRNA的表达;采用Western blot检测了奶牛输卵管上皮细胞输卵管蛋白的表达。同时,加入雌激素(10^(-10)mol/L)和butaprost(PE受体激动剂,10^(-6)mol/L),雌激素(10^(-10)mol/L)和fluprostenol(PF受体激动剂,10-6mol/L),分别观察对输卵管蛋白表达的影响。结果表明:雌激素与PGE_2受体对奶牛输卵管上皮细胞输卵管蛋白的分泌调控具有协同作用;雌激素与PGF_(2α)受体对奶牛输卵管上皮细胞输卵管蛋白的分泌调控具有间接拮抗作用。
In the present study,messenger RNA expressions of oviductal proteins were investigated using RT-PCR and protein levels were investigated using western blotting. The results indicated that after estradiol（ 10^-10 mol / L） and butaprost（ PE receptor agonists）（ 10^-6mol / L） treatment at the same time had synergy in oviductin expressions. After adding the estradiol（ 10^-10 mol / L） and fluprostenol（ PF receptor agonist）（ 10^-6mol / L） had indirect effects on oviductin expressions. The experimental results showed that estradiol and PGE2 receptors had synergistic roles on regulation of the expression of oviductin secretion in bovine oviduct epithelium cells. Our data suggested that estradiol and PGF2α receptors had indirect effects on oviductin expressions.
作者
张楠
黄娜
张莹
曹金山
ZHANG Nan HUANG Na ZHANG Ying CAO Jinshan(Inner Mongolia Agricultural University College of Veterina, Hohhot 010018, China)
出处
《畜牧与兽医》
北大核心
2017年第1期6-11,共6页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(3160490)
