摘要
目的探讨快速检测方法对呼吸道流感病毒、肺炎支原体检测的临床应用价值。方法选取该院2011年1月至2015年12月收集的386例上呼吸道感染患者标本进行检测,采用胶体金免疫沉积法检测患者鼻咽部分泌物中的A、B型流感病毒,采用快速培养法检测肺炎支原体,以间接免疫荧光法检测患者的血清病原体抗体IgM结果作为金标准,判断2种快速方法检测的临床价值。结果间接免疫荧光法共检出A型流感病毒感染患者54例,检出率13.59%;检出B型流感病毒感染患者37例,检出率9.59%;检出肺炎支原体感染患者61例,检出率15.80%。胶体金免疫沉积法检出A型流感病毒感染的敏感度为20.37%,特异性为92.77%;胶体金免疫沉积法检出B型流感病毒感染的敏感度为18.92%,特异性为93.41%;快速培养法检出肺炎支原体感染的敏感度为37.70%,特异性为89.85%。结论快速检测方法对呼吸道流感病毒、肺炎支原体检出的敏感度较低,临床上只能作为间接免疫荧光法检测的1种辅助手段。
Objective To explore the clinical application value of rapid detection method for respiratory tract influenza virus and mycoplasma pneumonia（MP）.Methods 386 cases were selected from patients with respiratory infections in our hospital from January 2011 to December 2015 in the study,nasopharyngeal secretions A,B influenza virus were detected by gold immunchromatographic assay,the Mycoplasma pneumoniae（MP）was detected by rapid culture,and pathogens-IgM were detected by immunofluorescence assay.In the end,the two rapid detection detection method were compared with immunofluorescence assay.Results 386 cases of respiratory tract infections patients were detected by immunofluorescence assay,54 cases were found influenza A virus infection,the positive rate was 13.59%,37 cases were found influenza B virus infection,the positive rate was 9.59%,61 cases were found MP infection,the positive rate was 15.80%.In detections of influenza A virus infection and influenza B virus infection,immunchromatographic assay sensitivity were 20.37%,18.92% and specificity were 93.41%,92.77%.In detection of MP infection,rapid culture sensitivity was 37.70% and specificity was 89.85%.Conclusion Rapid detection of respiratory influenza virus,MP infection sensitivity is low,only as a supplementary means of indirect immunofluorescence assay clinically.
作者
柴娟
CHAI Juan(Department of Clinical Laboratory, Hebei Provincial Hospital of Traditional Chinese Medicine, Shij iazhuang , Hebei 050011 ,China)
出处
《国际检验医学杂志》
CAS
2017年第2期179-181,共3页
International Journal of Laboratory Medicine
关键词
快速检测
流感病毒
肺炎支原体
rapid detection methods
influenza virus
Mycoplasma pneumoniae
