摘要
目的了解贵州省燃煤型砷中毒病区砷暴露者外周血中核因子E2相关因子2（Nff2）诱导的下游醌烟酰胺腺嘌呤二核苷酸磷酸（NADH）脱氢酶1（NQ01）和血红素氧合酶1（HO-1）mRNA及蛋白表达情况，探讨其在燃煤型砷中毒肝损伤发生发展中的作用。方法以贵州省兴仁县燃煤型砷中毒病区交乐村和长庆村为调查点，在健康体检基础上选择161例砷暴露者为砷暴露组，按照《地方性砷中毒诊断标准》（WS／T211-2001）分为病区非病例组（21例）和病例组（140例）。病例组参照《职业性中毒性肝病诊断标准》（GBZ59-2010）进一步分为轻度肝病组（52例）、中重度肝病组（36例），其余病例为无明显肝病组（52例）；同时以相邻的非病区村45例居民作为对照组；采集研究对象外周血，实时荧光定量PCR检测NQ01和HO-1 mRNA相对表达量，酶联免疫吸附试验（ELISA）检测NQ01和HO-1蛋白表达水平。结果①NQO1和HO-1 mRNA表达结果：随砷暴露人群肝损伤程度的加重，外周血NQO1和HO-1 mRNA相对表达量逐渐升高（F=5．548、10．961，P均〈0．05）。其中，轻度肝病组NQO1 mRNA相对表达量（中位数，0．918）高于对照组（0．576，P〈0．05），中重度肝病组NQO1 mRNA相对表达量（1．243）高于对照组、病区非病例组（0．653）、无明显肝病组（0．636）、轻度肝病组（P均〈0．05）；病区非病例组、无明显肝病组、轻度肝病组及中重度肝病组HO-1 mRNA相对表达量（1．059、1．225、11553、1．604）均高于对照组（0．767，P均〈0．05）；轻度肝病组HO-1 mRNA相对表达量高于病区非病例组（P〈0．05）；中重度肝病组HO-1 mRNA相对表达量高于病区非病例组、无明显肝病组（P均〈0．05）。②NQO1和HO-1蛋白表达结果：随砷暴露人群肝损伤程度的加重，血清中NQO1和HO-1蛋白表达水平逐渐升高（F=19．685、17．725，P均〈0．05）。�
Objective To detect the mRNA and protein expression of downstream quinine nicotinamide adenine dinucleotide （NADH） dehydrogenase 1 （NQO1） and heine oxygenase 1 （HO-1） induced by blood nuclearrelated factor E2 （Nrf2） in the peripheral blood of those exposed to arsenic in the endemic area of coal arsenicpoisoning in Guizhou Province, and to discuss its role in the process of occurrence and development of liver injury due to coal arsenic poisoning. Methods Jiaole and Changqing villages in coal-burning-borne arsenism areas in Xingren County of Guizhou were selected as the survey sites, and 161 cases of arsenic-exposed residents were selected as the arsenic exposed group based on physical examination. They were divided into non-patient group （21 cases） and patient group （140 cases） according to the ＂Diagnostic Criteria of Endemic Arsenism＂ （WS/T 211-2001）, and the patient group was further divided into mild hepatosis group （52 cases）, moderately severe hepatosis group （36 cases） and non-apparent hepatosis group （52 cases） according to the ＂Diagnostic Criteria of Occupational Chronic＂ （GBZ 59-2010）. Moreover, 45 residents from one village neighboring to non-epidemic area were selected as controls. Peripheral blood samples were collected from all subjects. And mRNA expression of NQO1 and HO-1 were detected by RT-qPCR. Content of NQQ1 and HO-1 were detected by enzyme linked immunosorbent assay （ELISA）. Results ①Results of mRNA expression of NQO1 and HO-1： the relative expression level of mRNA of NQO1 and HO-1 in peripheral blood went up gradually as the degree of liver injury of population exposed to arsenic increased （F = 5.548, 10.961, all P 〈 0.05）; thereinto, the relative expression level of mRNA of NQO1 of mild hepatosis group （median： 0.918） was higher than that of the control group （0.576, P 〈 0.05）, the relative expression level of mRNA of NQO1 of moderately severe hepatosis group （1.243） was higher than those of control group, non-
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2017年第1期21-25,共5页
Chinese Journal of Endemiology
基金
国家自然科学基金（81430077、81172603）
黔教合协同创新中心（[2014]06）
关键词
砷中毒
煤
肝
醌烟酰胺腺嘌呤二核苷酸磷酸脱氢酶1
血红素氧合酶1
氧化损伤
Arsenic poisoning
Coal
Liver
Quinine nicotinamide adenine dinucleotide dehydrogenase quinine 1
Heme oxygenase-1
Oxidative damage
