摘要
目的 观察贵州省燃煤污染型砷中毒病区砷暴露者外周血核因子E2相关因子2（Nrf2）与下游抗氧化反应元件（ARE）序列的结合能力（Nrf2-ARE结合能力）以及抗氧化酶基因的表达,为砷致机体肝氧化损伤机制的研究提供依据.方法 以贵州省兴仁县燃煤型砷中毒病区交乐村和长庆村为调查点,通过健康体检,选择161例砷暴露者为砷暴露组,按照《地方性砷中毒诊断标准》（WS/T 211-2001）分为病区非病例组（21例）和病例组（140例）,病例组参照《职业性中毒性肝病诊断标准》（GBZ 59-2010）进一步分为轻度肝病组（52例）、中重度肝病组（36例）,其余病例为无明显肝病组（52例）;同时选择相邻的非病区上坝田村45例居民作为对照组.采集观察对象外周血提取血细胞核蛋白：凝胶电泳迁移试验（EMSA）检测其外周血细胞核内Nrf2-ARE结合能力,实时荧光定量PCR检测铜锌超氧化物歧化酶（Cu/Zn-SOD）和谷胱甘肽过氧化物酶l（GSH-Px1） mRNA相对表达量.结果 Nrf2-ARE结合能力检测结果：各组人群外周血Nrf2-ARE结合能力组间比较差异有统计学意义（F=116.033,P＜0.05）.其中与对照组（3.14±1.34）比较,病区无明显肝病组（5.17±2.06）、轻度肝病组（13.13±4.84）及中重度肝病组（32.35±14.76） Nrf2-ARE结合能力均升高（P均＜0.05）;与病区非病例组（5.15±3.23）及无明显肝病组比较,轻度肝病组及中重度肝病组Nrf2-ARE结合能力升高（P均＜0.05）;与轻度肝病组比较,中重度肝病组Nrf2-ARE结合能力升高（P＜0.05）.Cu/Zn-SOD和GSH-Px1 mRNA表达结果：病区无明显肝病组、轻度肝病组及中重度肝病组Cu/Zn-SOD mRNA相对表达量[中位数（M）：1.127 8、1.257 8、1.6320]和GSH-Px1 mRNA相对表达量（M：1.334 5、1.940 9、2.062 6）均高于对照组（M：0.961 8、0.884 3）,组间比较差异均有统计学意义（x2=13.065、19.934,P均＜0.05）;轻度肝病组及�
Objective To detect the combining capacity of peripheral blood NF-E2-related factor 2 （Nrf2）of arsenic-exposed residents in the coal-contaminated arsenism area in Guizhou with the sequence of downstream antioxidant response element （ARE） as well as antioxidase gene expression,and to provide a basis for in-depth revelation of arsenic oxidative damage mechanism to human body.Methods Jiaole and Changqing villages in coal-burning-borne arsenism areas in Xingren County of Guizhou were selected as the survey spots,and 161 cases of arsenic-exposed residents were selected as the arsenic exposed group on the basis of physical examination.They were divided into non-patient group （21 cases） and patient group （140 cases） according to the ＂Diagnostic Criteria of Endemic Arsenism＂ （WS/T 211-2001）,and the patient group was further divided into mild hepatosis group （52 cases）,moderately severe hepatosis group （36 cases） and non-apparent hepatosis group （52 cases） according to the ＂Diagnostic Criteria of Occupational Chronic＂ （GBZ 59-2010）.Moreover,45 residents from one village neighboring to non-epidemic area were selected as controls.The hemocyte nucleoprotein was extracted from peripheral blood in the sampling subjects.The combining capacity of peripheral blood Nrf2-ARE was tested by electrophoretic mobility shift assay （EMSA）,and the relative expression quantity of Cu/Zn superoxide dismutase （Cu/Zn-SOD） and glutathione peroxidase 1 （GSH-Pxl） mRNA was tested with real-time fluorescence quantification PCR （qPCR）.Results The testing results of Nrf2-ARE combining capacity showed that the difference of Nrf2-ARE combining capacity between groups was statistically significant （F =116.033,P 〈 0.05）.Compared with the control group （3.14 ± 1.34）,the Nrf2-ARE combining capacity was higher in the non-apparent hepatosis group （5.17 ± 2.06）,mild hepatosis group （13.13 ± 4.84） and moderately severe hepatosis group （32.35 ± 14.76,all P 〈 0.05）;compared wi
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2015年第6期401-405,共5页
Chinese Journal of Endemiology
基金
国家自然科学基金（81172603、81430077）
关键词
砷中毒
煤
肝
氧化损伤
Arsenic poisoning
Coal
Liver
Oxidative damage
