摘要
为寻找一种简单、低成本及可规模化生产模式,以白鹤芋组培苗单株(2cm)为外植体,研究不同培养方法、接种量、通气量和糖种类对白鹤芋组培苗生长的影响。结果表明:利用接触式生物反应器培养,有利于白鹤芋丛生芽增殖,组培苗叶色浓绿,鲜重和干重高于其它处理。同时,在接种量60个单株、通气量为0.3vvm的处理中达到最佳效果。为大规模培养节约成本,糖种类选择了白糖。
In order to find a simple, low cost and large-scale production way for producing Spathiphyllum floribundum shoots,SpathiphyllumjToribundum shoot （2 cm） as explant, several factors affecting on the growth of SpathiphyllumyToribundum shoots were investigated. The results showed that raft culture in bioreactors was favorable for shoot growth with dark-green in leaf color, fresh weight and dry weight were higher than others. Meanwhile, 60 inoculation and air volume of 0.3 vvm were best for shoots prolifera- tion. It is a cost-saving way for large-scale culture when white sugar selected as source of carbon for the growth of SpathiphyllumyToribundum shoots.
作者
韩璐
修景润
代月
廉美兰
尹光浩
金美玉
HAN Lu XIU Jingrun DAI Yue LIAN Meilan YIN Guanhao JIN Meiyu(Agricultural College of Yanbian University, Yanji Jilin 133002,China Yanbian Academy of Agricultural Sciences, Longjing Jilin 133400,China Agro-Tech Extension and Service Center of Yanbian ,Yanji Jilin 133000,China)
出处
《延边大学农学学报》
2016年第4期313-316,共4页
Agricultural Science Journal of Yanbian University
基金
国家自然科学基金(30860176)
2016年大学生创新创业训练计划项目(ydbksky2016343)
关键词
白鹤芋
组培苗
接种量
通气量
生物反应器
Spathiphyllumfloribundum
tissue culture plants
inoculation density
air volume
Bioreactor
